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Chem Senses

Title:		"Immunolocalization of odorant-binding proteins in noctuid moths (Insecta, Lepidoptera)"
Author(s):		Zhang S; Maida R; Steinbrecht RA;
Address:		"Max-Planck-Institut fur Verhaltensphysiologie, D-82319 Seewiesen, Germany"
Journal Title:		Chem Senses
Year:		2001
Volume:		26
Issue:		7
Page Number:		885 - 896
DOI:		10.1093/chemse/26.7.885
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"Odorant-binding proteins were studied in the noctuid moths Agrotis segetum, Autographa gamma, Helicoverpa armigera, Heliothis virescens and Spodoptera littoralis using antisera raised against the pheromone-binding protein (PBP) and general odorant-binding protein 2 (GOBP2) of Antheraea polyphemus (Saturniidae). Proteins immunoreacting with these antisera were only found on the antennae and PBP and GOBP2 could be identified on western blots of males and females of all five species. PBPs were predominantly localized in sensilla trichodea and GOBP2 in sensilla basiconica, in good correlation with the stimulus specificity of the receptor cells in these sensilla. In H. armigera and H. virescens the majority of the s. trichodea immunoreacted with the antiserum against PBP of A. polyphemus; in A. segetum, A. gamma and S. littoralis, on the other hand, a high percentage of s. trichodea remained unlabelled. Probably, the PBP expressed in these sensilla is so different that it does not immunoreact with the antiserum used. Such a protein was found by native PAGE of antennal extracts of A. segetum and S. littoralis. These data correlate with the fact that the two heliothine species use pheromones with the same alkyl chain length as A. polyphemus, while the other three species use pheromones with shorter chains. In H. armigera, H. virescens, A. gamma and S. littoralis female antennae were also immunolabelled and a large number of PBP-expressing s. trichodea was consistently found. In S.littoralis this fits with the electrophysiologically recorded high pheromone sensitivity of female s. trichodea, whereas in females of H. armigera and H. virescens no or only weak responses to pheromone stimulation have been reported. Therefore, PBP expression in a sensillum does not necessarily imply pheromone sensitivity of its receptor cells"
Keywords:		"Animals Blotting, Western Carrier Proteins/biosynthesis Electrophoresis, Polyacrylamide Gel Female Immunoblotting Immunohistochemistry *Insect Proteins Male Mechanoreceptors/*metabolism Moths Pheromones/metabolism Protein Binding Receptors, Odorant/*biosy;"
Notes:		"MedlineZhang, S Maida, R Steinbrecht, R A eng Research Support, Non-U.S. Gov't England 2001/09/14 Chem Senses. 2001 Sep; 26(7):885-96. doi: 10.1093/chemse/26.7.885"