« Previous AbstractIdentification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower    Next Abstract"Melatonin ameliorates ochratoxin A induced liver inflammation, oxidative stress and mitophagy in mice involving in intestinal microbiota and restoring the intestinal barrier function" »

J Nutr

Title:		"l-Arginine Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Ovine Intestinal Epithelial Cells by Regulating Apoptosis, Mitochondrial Function, and Autophagy"
Author(s):		Zhang H; Liu X; Fan Y; Yu Y; Loor JJ; Elsabagh M; Peng A; Wang H;
Address:		"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou, China. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, China. Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, USA. Department of Animal Production and Technology, Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, Nigde, Turkey. Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt"
Journal Title:		J Nutr
Year:		2021
Volume:		151
Issue:		4
Page Number:		1038 - 1046
DOI:		10.1093/jn/nxaa428
ISSN/ISBN:		1541-6100 (Electronic) 0022-3166 (Linking)
Abstract:		"BACKGROUND: Previous studies demonstrated that dietary l-arginine (Arg) alters the equilibrium between reactive oxygen species (ROS) generation and biological defenses to resist oxidant-induced toxicity. Whether supplying Arg can protect ovine intestinal epithelial cells (OIECs) from hydrogen peroxide (H2O2)-induced oxidative damage is unclear. OBJECTIVES: The current study aimed to examine the effect of Arg on mitophagy, mitochondrial dysfunction, and apoptosis induced by H2O2 in OIECs. METHODS: The OIECs were incubated in Arg-free DMEM supplemented with 100 muM Arg (CON) or 350 muM Arg (ARG) alone or with 150 muM H2O2 (CON + H2O2, ARG + H2O2) for 24 h. Cellular apoptosis, mitochondrial function, autophagy, and the related categories of genes and proteins were determined. All data were analyzed by ANOVA using the general linear model procedures of SAS (SAS Institute) for a 2 x 2 factorial design. RESULTS: Relative to the CON and ARG groups, H2O2 administration resulted in 44.9% and 26.5% lower (P < 0.05) cell viability but 34.7% and 61.8% greater (P < 0.05) ROS concentration in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 40.7% and 28.8% lower (P < 0.05) ROS concentration but 14.9%-49.0% and 29.3%-64.1% greater (P < 0.05) mitochondrial membrane potential, relative mitochondrial DNA content, and complex (I-IV) activity in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 33.9%-53.1% and 22.4%-49.1% lower (P < 0.05) mRNA abundance of proapoptotic genes, respectively. Relative to the CON and CON + H2O2 groups, Arg supplementation resulted in 33.0%-59.2% and 14.6%-37.7% lower (P < 0.05) abundance of proapoptotic, mitophagy, and cytoplasmic cytochrome c protein, respectively. CONCLUSIONS: Supply of Arg protects OIECs against H2O2-induced damage partly by improving mitochondrial function and alleviating cellular apoptosis and autophagy"
Keywords:		"Adenosine Triphosphate/metabolism Animals Apoptosis/drug effects Arginine/*pharmacology Autophagy/drug effects Cells, Cultured DNA, Mitochondrial/metabolism Dietary Supplements Hydrogen Peroxide/toxicity Intestinal Mucosa/*drug effects/metabolism/patholog;"
Notes:		"MedlineZhang, Hao Liu, Xiaoyun Fan, Yaotian Yu, Yin Loor, Juan J Elsabagh, Mabrouk Peng, Along Wang, Hongrong eng Research Support, Non-U.S. Gov't 2021/03/12 J Nutr. 2021 Apr 8; 151(4):1038-1046. doi: 10.1093/jn/nxaa428"