| Title: | Common and unique cis-acting elements mediate xanthotoxin and flavone induction of the generalist P450 CYP321A1 |
| Author(s): | Zhang C; Wong A; Zhang Y; Ni X; Li X; |
| Address: | "1] Key Laboratory of Plant Protection Resources and Pest Integrated Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China [2] Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA. Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA. Key Laboratory of Plant Protection Resources and Pest Integrated Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China. USDA-ARS Crop Genetics and Breeding Research Unit, Tifton, GA 31793, USA" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "How polyphagous herbivores up-regulate their counterdefense genes in response to a broad range of structurally different allelochemicals remains largely unknown. To test whether this is accomplished by having more allelochemical-response elements or the similar number of functionally more diverse elements, we mapped out the cis-acting elements mediating the induction of the allelochemical-metabolizing CYP321A1 from the generalist Helicoverpa zea by xanthotoxin and flavone, two structurally distinct allelochemicals with very different encounter rate by this species. Seven xanthotoxin-responsive elements were localized by analyzing promoter activities of varying length of CYP321A1 promoter in H. zea fatbody cells. Compared with the 5 flavone-responsive elements mapped out previously, there are four common elements (1 essential element, 2 enhancers, and 1 negative element) mediating induction of CYP321A1 by both of the two allelochemicals. The remaining four elements (3 enhancers and 1 negative element), however, only regulate induction of CYP321A1 by either of the two allelochemicals. Co-administration of the two allelochemicals resulted in an induction fold that is significantly lower than the expected additive value of the two allelochemicals. These results indicate that xanthotoxin- and flavone-induced expressions of CYP321A1 are mediated mainly by the functionally more diverse common elements although the allelochemical-unique elements also play a role" |
| Keywords: | Amino Acid Sequence/genetics Animals Cytochrome P-450 Enzyme System/biosynthesis/*genetics Flavones/*pharmacology Gene Expression Regulation/drug effects Insecticides/administration & dosage/pharmacology Lepidoptera/drug effects/*enzymology Methoxsalen/*p; |
| Notes: | "MedlineZhang, Chunni Wong, Andrew Zhang, Yalin Ni, Xinzhi Li, Xianchun eng Research Support, Non-U.S. Gov't England 2014/09/30 Sci Rep. 2014 Sep 29; 4:6490. doi: 10.1038/srep06490" |
