Title: | Elucidation of ( Z)-3-Hexenyl-beta-glucopyranoside Enhancement Mechanism under Stresses from the Oolong Tea Manufacturing Process |
Author(s): | Zeng L; Wang X; Xiao Y; Gu D; Liao Y; Xu X; Jia Y; Deng R; Song C; Yang Z; |
Address: | "Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany , South China Botanical Garden, Chinese Academy of Sciences , No. 723 Xingke Road, Tianhe District , Guangzhou 510650 , China. College of Advanced Agricultural Sciences , University of Chinese Academy of Sciences , No.19A Yuquan Road , Beijing 100049 , China. State Key Laboratory of Tea Plant Biology and Utilization , Anhui Agricultural University , No. 130 Changjiang West , Hefei 230036 , China" |
ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
Abstract: | "The enzymatic hydrolysis of glycosidically bound volatiles (GBVs) plays an important role in tea aroma formation during the tea manufacturing process. However, during the enzyme-active manufacturing process of oolong tea, most GBVs showed no reduction, while ( Z)-3-hexenyl-beta-glucopyranoside significantly enhanced at the turnover stage. This study aimed to determine the reason for this increase in ( Z)-3-hexenyl-beta-glucopyranoside. Continuous wounding stress in the turnover stage did not enhance the expression level of glycosyltransferase 1 ( CsGT1), while it induced a significant increase in the ( Z)-3-hexenol content ( p = 0.05). Furthermore, observing the cell structures of tea leaves exposed to continuous wounding and subcellular localizations of CsGTs suggested that the interaction of ( Z)-3-hexenol (substrate) and CsGT1 (enzyme) was available. In conclusion, both continuous wounding and subcellular localizations led to a ( Z)-3-hexenyl-beta-glucopyranoside enhancement mechanism during the oolong tea's turnover stage. These results advance our understanding of GBV formation during the tea manufacturing process and their relationship with the stress from the tea manufacturing process. In addition, the information will help us further evaluate contribution of GBVs to enzymatic formation of oolong tea aroma compounds" |
Keywords: | "Camellia sinensis/*chemistry/enzymology/genetics/physiology Food Handling/*methods Glucosides/*analysis Glycosyltransferases/genetics/metabolism Plant Leaves/chemistry/physiology Plant Proteins/genetics/metabolism Stress, Physiological Volatile Organic Co;" |
Notes: | "MedlineZeng, Lanting Wang, Xiaoqin Xiao, Yangyang Gu, Dachuan Liao, Yinyin Xu, Xinlan Jia, Yongxia Deng, Rufang Song, Chuankui Yang, Ziyin eng Evaluation Study 2019/05/28 J Agric Food Chem. 2019 Jun 12; 67(23):6541-6550. doi: 10.1021/acs.jafc.9b02228. Epub 2019 Jun 3" |