| Title: | Influence of dietary aconitine and nicotine on the gut microbiota of two lepidopteran herbivores |
| Author(s): | Zeng JY; Wu DD; Shi ZB; Yang J; Zhang GC; Zhang J; |
| Address: | "Department of Forest Protection, School of Forestry, Northeast Forestry University, Harbin, China. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "The gut microbiota plays an important role in pheromone production, pesticide degradation, vitamin synthesis, and pathogen prevention in the host animal. Therefore, similar to gut morphology and digestive enzyme activity, the gut microbiota may also get altered under plant defensive compound-induced stress. To test this hypothesis, Dendrolimus superans larvae were fed either aconitine- or nicotine-treated fresh leaves of Larix gmelinii, and Lymantria dispar larvae were fed either aconitine- or nicotine-treated fresh leaves of Salix matsudana. Subsequently, the larvae were sampled 72hr after diet administration and DNA extracted from larval enteric canals were employed for gut microbial 16S ribosomal RNA gene sequencing (338 F and 806 R primers). The sequence analysis revealed that dietary nicotine and aconitine influenced the dominant bacteria in the larval gut and determined their abundance. Moreover, the effect of either aconitine or nicotine on D. superans and L. dispar larvae had a greater dependence on insect species than on secondary plant metabolites. These findings further our understanding of the interaction between herbivores and host plants and the coevolution of plants and insects" |
| Keywords: | "Aconitine/*pharmacology Animals Bacteria/classification/genetics Gastrointestinal Microbiome/*drug effects Larix Larva/drug effects/microbiology Moths/drug effects/growth & development/*microbiology Nicotine/*pharmacology Plant Leaves RNA, Ribosomal, 16S;" |
| Notes: | "MedlineZeng, Jian-Yong Wu, De-Dong Shi, Zhong-Bin Yang, Jing Zhang, Guo-Cai Zhang, Jie eng 2572017AA18/Fundamental Research Funds for Chinese Central Universities/ [2019]HZT02 and/Forestry Science and Technology Promotion Demonstration Fund Project of Chinese Central Finance/ JLT[2016] 13/Forestry Science and Technology Promotion Demonstration Fund Project of Chinese Central Finance/ 2020/04/24 Arch Insect Biochem Physiol. 2020 Jul; 104(3):e21676. doi: 10.1002/arch.21676. Epub 2020 Apr 23" |
