| Title: | HPLC and ELISA analyses of larval bile acids from Pacific and western brook lampreys |
| Author(s): | Yun SS; Scott AP; Bayer JM; Seelye JG; Close DA; Li W; |
| Address: | "Department of Fisheries and Wildlife, Michigan State University, 13 Natural Resources Building, East Lansing, MI 48824, USA" |
| DOI: | 10.1016/s0039-128x(03)00088-6 |
| ISSN/ISBN: | 0039-128X (Print) 0039-128X (Linking) |
| Abstract: | "Comparative studies were performed on two native lamprey species, Pacific lamprey (Lampetra tridentata) and western brook lamprey (Lampetra richardsoni) from the Pacific coast along with sea lamprey (Petromyzon marinus) from the Great Lakes, to investigate their bile acid production and release. HPLC and ELISA analyses of the gall bladders and liver extract revealed that the major bile acid compound from Pacific and western brook larval lampreys was petromyzonol sulfate (PZS), previously identified as a migratory pheromone in larval sea lamprey. An ELISA for PZS has been developed in a working range of 20 pg-10 ng per well. The tissue concentrations of PZS in gall bladder were 127.40, 145.86, and 276.96 micro g/g body mass in sea lamprey, Pacific lamprey, and western brook lamprey, respectively. Releasing rates for PZS in the three species were measured using ELISA to find that western brook and sea lamprey released PZS 20 times higher than Pacific lamprey did. Further studies are required to determine whether PZS is a chemical cue in Pacific and western brook lampreys" |
| Keywords: | "Animals Bile Acids and Salts/*analysis/metabolism Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay Gallbladder/chemistry Lampreys/*metabolism/physiology Larva/chemistry Liver/chemistry Pheromones/analysis/metabolism;" |
| Notes: | "MedlineYun, Sang-Seon Scott, Alexander P Bayer, Jennifer M Seelye, James G Close, David A Li, Weiming eng Comparative Study Research Support, Non-U.S. Gov't 2003/08/09 Steroids. 2003 Aug; 68(6):515-23. doi: 10.1016/s0039-128x(03)00088-6" |
