| Title: | Mating-type factor-specific regulation of the fumagillin/pseurotin secondary metabolite supercluster in Aspergillus fumigatus |
| Author(s): | Yu Y; Blachowicz A; Will C; Szewczyk E; Glenn S; Gensberger-Reigl S; Nowrousian M; Wang CCC; Krappmann S; |
| Address: | "Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitatsklinikum Erlangen and Friedrich-Alexander-Universitat Erlangen-Nurnberg, Germany. School of Pharmacy, John Staffer Pharmaceutical Sciences Center, University of Southern California, Los Angeles, CA, USA. Research Center for Infectious Diseases, Julius-Maximilians-Universitat Wurzburg, Germany. Henriette Schmidt-Burkhardt Chair of Food Chemistry, Emil Fischer Center, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Germany. Department of General and Molecular Botany, Ruhr University Bochum, Germany" |
| ISSN/ISBN: | 1365-2958 (Electronic) 0950-382X (Linking) |
| Abstract: | "In the human pathogenic mold Aspergillus fumigatus, sexual identity is determined by the mating-type idiomorphs MAT1-1 and MAT1-2 residing at the MAT locus. Upon crossing of compatible partners, a heterothallic mating is executed to eventually form cleistothecia that contain recombinant ascospores. Given that the MAT1 gene products are DNA binding master regulators that govern this complex developmental process, we monitored the MAT1-driven transcriptomes of A. fumigatus by conditional overexpression of either MAT1 gene followed by RNA-seq analyses. Numerous genes related to the process of mating were found to be under transcriptional control, such as pheromone production and recognition. Substantial differences between the MAT1-1- and MAT1-2-driven transcriptomes could be detected by functional categorization of differentially expressed genes. Moreover, a significant and distinct impact on expression of genetic clusters of secondary metabolism became apparent, which could be verified on the product level. Unexpectedly, specific cross-regulation of the fumagillin/pseurotin supercluster was evident, thereby uncoupling its co-regulatory characteristic. These insights imply a tight interconnection of sexual development accompanied by ascosporogenesis with secondary metabolite production of a pathogenic fungus and impose evolutionary constraints that link these two fundamental aspects of the fungal lifestyle" |
| Keywords: | "*Aspergillus fumigatus/genetics/metabolism *Cyclohexanes/metabolism *Fatty Acids, Unsaturated/genetics/metabolism Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal Genes, Mating Type, Fungal *Mating Factor/genetics/metabolism Multigen;" |
| Notes: | "MedlineYu, Yidong Blachowicz, Adriana Will, Cornelia Szewczyk, Edyta Glenn, Steven Gensberger-Reigl, Sabrina Nowrousian, Minou Wang, Clay C C Krappmann, Sven eng 15, 2015-1/Bavarian Californian Technology Center/International 12-08-17-1/ELAN Fonds of the University of Erlangen-Nurnberg/International KR 2294/3-1/Deutsche Forschungsgemeinschaft/International NO407/7-1/Deutsche Forschungsgemeinschaft/International 031A408A/Bundesministerium fur Bildung und Forschung/International 2294/University of Wisconsin-Madison/International 3-1/University of Wisconsin-Madison/International NO407/University of Wisconsin-Madison/International 7-1/University of Wisconsin-Madison/International 031A408A/University of Wisconsin-Madison/International 12-08-17-1/University of Wisconsin-Madison/International German Research Foundation/International Ministry of Education/International 031A408A/German Federal Ministry of Education and Research/International KR 2294/3-1/German Research Foundation/International NO407/7-1/German Research Foundation/International Research Support, Non-U.S. Gov't England 2018/09/22 Mol Microbiol. 2018 Dec; 110(6):1045-1065. doi: 10.1111/mmi.14136. Epub 2018 Oct 23" |
