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Proc Natl Acad Sci U S A

Title:		Guanylyl cyclase expression in specific sensory neurons: a new family of chemosensory receptors
Author(s):		Yu S; Avery L; Baude E; Garbers DL;
Address:		"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1997
Volume:		94
Issue:		7
Page Number:		3384 - 3387
DOI:		10.1073/pnas.94.7.3384
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"A guanylyl cyclase (GC-D) was recently shown to be expressed in a subclass of neurons within the neuroepithelim of the rat, but given that only a single cyclase was discovered, whether it represents an odorant/pheromone receptor as has been suggested for the large family of seven-transmembrane receptors remains unclear. Through cloning and expression of cDNA we now demonstrate that at least 29 genomic or cDNA sequences found in Caenorhabditis elegans represent guanylyl cyclases. Many of the membrane forms retain cysteine residues conserved within the extracellular, ligand-binding domain of known cyclase receptors. Of eight orphan cyclase receptor::GFP (green fluroescence protein) fusion constructs for which signals were obtained, all were expressed in specific sensory neurons. Furthermore, a cyclase/GFP fusion protein (GCY-10/GFP) was principally expressed in the sensory cilium, suggesting these cyclases function as primary chemosensory receptors. For the first time, we also found that chemosensory neurons (ASE), known to be bilaterally symmetric, demonstrate absolute right or left sidedness with respect to the expression of three different cyclases. Thus, the guanylyl cyclases represent an unexpectedly large and new family of sensory neuron receptors that may complement the 7-transmembrane family of odorant/pheromone receptors"
Keywords:		"Animals COS Cells Caenorhabditis elegans/genetics Chemoreceptor Cells/*metabolism Cloning, Molecular DNA, Complementary Guanylate Cyclase/*genetics Neurons, Afferent/*enzymology Rats Sensory Receptor Cells/*metabolism Transgenes;"
Notes:		"MedlineYu, S Avery, L Baude, E Garbers, D L eng R01 HL046154/HL/NHLBI NIH HHS/ 1997/04/01 Proc Natl Acad Sci U S A. 1997 Apr 1; 94(7):3384-7. doi: 10.1073/pnas.94.7.3384"