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Methods Mol Biol

Title:		Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2
Author(s):		Yu CR;
Address:		"Stowers Institute for Medical Research, Kansas City, MO, USA"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		211 - 220
DOI:		10.1007/978-1-62703-619-1_15
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Print) 1064-3745 (Linking)
Abstract:		The vomeronasal organ (VNO) in vertebrate animals detects pheromones and interspecies chemical signals. We describe in this chapter a Ca(2+) imaging approach using transgenic mice that express the genetically encoded Ca(2+) sensor G-CaMP2 in VNO tissue. This approach allows us to analyze the complex patterns of the vomeronasal neuron response to large number of chemosensory stimuli
Keywords:		"Animals Calcium/chemistry Calmodulin/genetics Green Fluorescent Proteins/genetics Intracellular Calcium-Sensing Proteins/biosynthesis/genetics/*metabolism Mice Mice, Transgenic Myosin-Light-Chain Kinase/genetics Olfactory Mucosa/innervation/metabolism Pep;"
Notes:		"MedlineYu, C Ron eng R01 DC008003/DC/NIDCD NIH HHS/ DC 008003/DC/NIDCD NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2013/09/10 Methods Mol Biol. 2013; 1068:211-20. doi: 10.1007/978-1-62703-619-1_15"