| Title: | Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device |
| Author(s): | You JB; Lee B; Choi Y; Lee CS; Peter M; Im SG; Lee SS; |
| Address: | "Department of Chemical & Materials Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada. Department of Chemical Engineering & Applied Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea. Department of Chemical & Biomolecular Engineering, KAIST, Daejeon, 34141, Republic of Korea. Institute for Biochemistry, ETH Zurich, Zurich, CH 8093, Switzerland. Scientific Center for Optical & Electron Microscopy (ScopeM), ETH Zurich, Zurich, CH 8093, Switzerland" |
| ISSN/ISBN: | 1940-9818 (Electronic) 0736-6205 (Linking) |
| Abstract: | "Poly(dimethylsiloxane) (PDMS) is widely used as a microfluidics platform material; however, it absorbs various molecules, perturbing specific chemical concentrations in microfluidic channels. We present a simple solution to prevent adsorption into a PDMS microfluidic device. We used a vapor-phase-deposited nanoadhesive layer to seal PDMS microfluidic channels. Absorption of fluorescent molecules into PDMS was efficiently prevented in the nanolayer-treated PDMS device. Importantly, when cultured in a nanolayer-treated PDMS device, yeast cells exhibited the expected concentration-dependent response to a mating pheromone, including mating-specific morphological and gene expression changes, while yeast cultured in an untreated PDMS device did not properly respond to the pheromone. Our method greatly expands microfluidic applications that require precise control of molecule concentrations" |
| Keywords: | PDMS microfluidics device bonding nanoadhesive layer protein absorption yeast mating; |
| Notes: | "PubMed-not-MEDLINEYou, Jae Bem Lee, Byungjin Choi, Yunho Lee, Chang-Soo Peter, Matthias Im, Sung Gap Lee, Sung Sik eng Research Support, Non-U.S. Gov't England 2020/05/07 Biotechniques. 2020 Jul; 69(1):404-409. doi: 10.2144/btn-2020-0025. Epub 2020 May 6" |
