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Pestic Biochem Physiol

Title:		Binding properties of chemosensory protein 12 in Riptortus pedestris to aggregation pheromone (E)-2-hexenyl (Z)-3-hexenoate
Author(s):		Yin MZ; Li JQ; Liu Q; Ma S; Hu ZZ; Liu XZ; Wang CW; Yao WC; Zhu XY; Wang YY; Li JB; Zhang YN;
Address:		"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China; Suzhou Academy of Agricultural Sciences, Suzhou 234000, China. Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China. Suzhou Academy of Agricultural Sciences, Suzhou 234000, China. Suzhou Academy of Agricultural Sciences, Suzhou 234000, China. Electronic address: 1399183945@qq.com. Suzhou Academy of Agricultural Sciences, Suzhou 234000, China; Suzhou Vocational and Technical College, Suzhou 234000, China. Electronic address: lijinbu0991@163.com. Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China. Electronic address: ynzhang_insect@163.com"
Journal Title:		Pestic Biochem Physiol
Year:		2023
Volume:		20230701
Issue:
Page Number:		105513 -
DOI:		10.1016/j.pestbp.2023.105513
ISSN/ISBN:		1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:		"Riptortus pedestris (bean bug), a common soybean pest, has a highly developed olfactory system to find hosts for feeding and oviposition. Chemosensory proteins (CSPs) have been identified in many insect species; however, their functions in R. pedestris remain unknown. In this study, quantitative real time-polymerase chain reaction (qRT-PCR) revealed that the expression of RpedCSP12 in the adult antennae of R. pedestris increased with age. Moreover, a significant difference in the expression levels of RpedCSP12 was observed between male and female antennae at one and three days of age. We also investigated the binding ability of RpedCSP12 to different ligands using a prokaryotic expression system and fluorescence competitive binding assays. We found that RpedCSP12 only bound to one aggregation pheromone, (E)-2-hexenyl (Z)-3-hexenoate, and its binding decreased with increasing pH. Furthermore, homology modelling, molecular docking, and site-directed mutagenesis revealed that the Y27A, L74A, and L85A mutants lost their binding ability to (E)-2-hexenyl (Z)-3-hexenoate. Our findings highlight the olfactory roles of RpedCSP12, providing insights into the mechanism by which RpedCSPs bind to aggregation pheromones. Therefore, our study can be used as a theoretical basis for the population control of R. pedestris in the future"
Keywords:		Animals Female *Pheromones Molecular Docking Simulation *Heteroptera/genetics Soybeans Aggregation pheromone Chemosensory protein Fluorescence competitive binding assays Riptortus pedestris Site-directed mutagenesis;
Notes:		"MedlineYin, Mao-Zhu Li, Jian-Qiao Liu, Qiang Ma, Sai Hu, Zhuang-Zhuang Liu, Xing-Zhou Wang, Chao-Wei Yao, Wei-Chen Zhu, Xiu-Yun Wang, Yue-Ying Li, Jin-Bu Zhang, Ya-Nan eng 2023/08/03 Pestic Biochem Physiol. 2023 Aug; 194:105513. doi: 10.1016/j.pestbp.2023.105513. Epub 2023 Jul 1"