| Title: | Exploratory study on noninvasive biomarker of silicosis in exhaled breath by solid-phase microextraction-gas chromatography-mass spectrometry analysis |
| Author(s): | Yi Z; Dong S; Wang X; Xu M; Li Y; Xie L; |
| Address: | "West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China. Chengdu Center for Disease Control and Prevention, Chengdu, 610066, China. West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China. lyxlee2008@hotmail.com. Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China. lyxlee2008@hotmail.com. West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China. linshenxie@163.com" |
| Journal Title: | Int Arch Occup Environ Health |
| DOI: | 10.1007/s00420-023-01971-y |
| ISSN/ISBN: | 1432-1246 (Electronic) 0340-0131 (Linking) |
| Abstract: | "BACKGROUND: As a chronic occupational disease, silicosis could cause irreversible and incurable impair to the lung. The current diagnosis of silicosis relies on imaging of X-ray or CT, but these methods cannot detect lung lesions in the early stage of silicosis. OBJECTIVE: To establish a regular screening and early diagnosis methods for silicosis, which could be helpful for the prevention and treatment of silicosis. METHODS: A total of 161 subjects were enrolled in the study, including 69 patients with silicosis (SILs) and 92 healthy controls. The exhaled breath samples of the subjects were collected with breath sampler and Tedlar bag. The analysis of volatile organic compounds (VOCs) in exhaled breath was performed by solid-phase microextraction (SPME) combined with gas chromatography mass spectrometry (GC-MS). RESULTS: After excluding the pollutants from sampling bags and instruments, 86 VOCs have been identified in the exhaled breath. The orthogonal partial least squares-discriminant analysis (OPLS-DA) was employed for the screening of potential biomarkers of silicosis. Those components that related to smoking were also excluded from the biomarkers. Finally, nine possible biomarkers for silicosis were screened out, including 2,3-butanedione, ethyl acetate, chlorobenzene, o-cymene, 4-ethylhex-2-ynal, 3,5-dimethyl-3-heptanol, hydroquinone, phthalic anhydride and 5-(2-methylpropyl)nonane. Based on these biomarkers screened, a predicted model for silicosis was generated with the accuracy of 89.61%. CONCLUSION: The nine biomarkers in exhaled breath were preliminarily screened out for the early diagnosis of silicosis, which can be helpful to the establishment of a noninvasive screening method for silicosis. Follow-up studies should be conducted to further verify these markers" |
| Keywords: | Humans Gas Chromatography-Mass Spectrometry/methods Solid Phase Microextraction Breath Tests/methods Biomarkers/analysis *Silicosis/diagnosis *Volatile Organic Compounds/analysis Biomarker Exhaled breath Silicosis Volatile organic compounds; |
| Notes: | "MedlineYi, Zonghui Dong, Simin Wang, Xixi Xu, Mucen Li, Yongxin Xie, Linshen eng 2021YFS0182/Science and Technology Department of Sichuan Province/ Germany 2023/04/18 Int Arch Occup Environ Health. 2023 Aug; 96(6):857-868. doi: 10.1007/s00420-023-01971-y. Epub 2023 Apr 17" |
