| Title: | The Desaturase Gene Nlug-desatA2 Regulates the Performance of the Brown Planthopper Nilaparvata lugens and Its Relationship with Rice |
| Author(s): | Ye W; Zeng J; Hu W; Bustos-Segura C; Noman A; Lou Y; |
| Address: | "State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China. Laboratory of Fundamental and Applied Research in Chemical Ecology, University of Neuchatel, 2000 Neuchatel, Switzerland. Department of Botany, Government College University, Faisalabad 38040, Pakistan" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Insect desaturases are known to play an important role in chemical communication between individuals. However, their roles in insect growth, development and fecundity, and in regulating interactions of insects with plants, remain largely unknown. In this study, we explored the functions of Nlug-desatA2, a desaturase gene of the brown planthopper (BPH), Nilaparvata lugens (Stal). The RNA interference-based knockdown of Nlug-desatA2 decreased the ratio of monounsaturated fatty acids to saturated fatty acids, and the level of fatty acids and triglycerides in BPH. Nlug-desatA2-knockdown also reduced the food intake, body mass and fecundity of female BPH adults, and led to abdomen atrophy and ovarian agenesis. Nlug-desatA2-knockdown suppressed the transcription of TOR (target of rapamycin), Lpp (Lipophorin) and AKHR (adipokinetic hormone receptor) in female adults. Moreover, the corrected survival rate of BPH with Nlug-desatA2-knockdown fed an artificial diet was higher than the survival rate of those fed on rice plants. Higher levels of salicylic acid in rice infested by Nlug-desatA2-knockdown female BPH adults than in rice infested by control BPH may be the reason. These findings demonstrate that Nlug-desatA2 has an essential role in lipid metabolism and is involved in the food intake, survival, development and fecundity of BPH. In addition, this gene is likely involved in regulating the responses of rice to BPH infestation" |
| Keywords: | Animals Eating Fatty Acid Desaturases/*genetics/metabolism Female Fertility Gene Knockdown Techniques Hemiptera/genetics/*physiology Herbivory Insect Proteins/*genetics/metabolism Lipid Metabolism/genetics *Oryza/metabolism Ovary/growth & development Sali; |
| Notes: | "MedlineYe, Wenfeng Zeng, Jiamei Hu, Wenhui Bustos-Segura, Carlos Noman, Ali Lou, Yonggen eng 31520103912/National Natural Science Foundation of China/ CARS-01-40/Earmarked Fund for China Agriculture Research System/ Switzerland 2020/06/14 Int J Mol Sci. 2020 Jun 10; 21(11):4143. doi: 10.3390/ijms21114143" |
