| Title: | Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles |
| Author(s): | Yao R; Zhao M; Zhong L; Li Y; Li D; Deng Z; Ma X; |
| Address: | "National Center for Citrus Improvement (Changsha), Hunan Agricultural University, Changsha, People's Republic of China. College of Horticulture, Hunan Agricultural University, Changsha, People's Republic of China. Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Bactrocera minax, one of the most important citrus pests, oviposits exclusively on citrus fruit. In the insect olfactory system, odorant-binding proteins (OBPs) facilitate the initial recognition role of host odor molecules. The aim of this study was to characterize the functional OBPs of B. minax and identify specific volatile organic compounds in the Citrus genus as OBP targets. RESULTS: BminOBP9 (BminGOBP99a), a closely related homolog of BdorGOBP99a, which reduces the egg-laying behavior of Bactrocera dorsalis through silencing technology, was cloned, expressed, and purified. The binding ability of BminOBP9 to 11 citrus volatiles was then examined using fluorescence competition binding assays (FCBA). The results demonstrated that BminOBP9 could bind to all tested citrus volatiles, as could BdorGOBP99a, ZcucGOBP99a, and ZtauGOBP99a. Interestingly, the binding ability of BminOBP9 was the strongest among the four, suggesting that BminOBP9 may have a function in the specific recognition of citrus volatiles. Furthermore, we aligned the above four proteins and found nine distinctive amino acid sites in BminOBP9. To identify the unique binding sites of BminOBP9, we produced the nine mutants using site-directed mutagenesis. Further FCBA showed that the binding ability of the nine mutants to citrus volatiles significantly reduced, and six of them (substitutes S24P, L36F, E53K, N68D, D112A, and S118R) had the weakest binding ability. CONCLUSION: The results demonstrated that BminOBP9 was the specific protein involved in the perception of citrus host volatiles by B. minax. Moreover, BminOBP9 could prove efficient in screening the candidate odors for pest management. (c) 2020 Society of Chemical Industry" |
| Keywords: | "Animals Carrier Proteins *Citrus/metabolism Insect Proteins/genetics/metabolism Insecta/metabolism Odorants Protein Binding *Receptors, Odorant/genetics/metabolism *Tephritidae/genetics/metabolism Bactrocera minax citrus odorant-binding protein volatile o;" |
| Notes: | "MedlineYao, Runxian Zhao, Mingming Zhong, Ling Li, Yi Li, Dazhi Deng, Ziniu Ma, Xianfeng eng 2017YFD0202000/National Key R&D Program of China/ 2019RS1052/The Science & Technology Innovation Program of Hunan/ CX2017B358/The Scientific Research Innovation of Hunan Province Graduate Project/ England 2020/10/10 Pest Manag Sci. 2021 Mar; 77(3):1214-1225. doi: 10.1002/ps.6132. Epub 2020 Oct 21" |
