| Title: | MYB21 interacts with MYC2 to control the expression of terpene synthase genes in flowers of Freesia hybrida and Arabidopsis thaliana |
| Author(s): | Yang Z; Li Y; Gao F; Jin W; Li S; Kimani S; Yang S; Bao T; Gao X; Wang L; |
| Address: | "Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun, China. Department of Biological and Physical Sciences, Karatina University, Karatina, Kenya. National Demonstration Center for Experimental Biology Education, Northeast Normal University, Changchun, China" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Linking) |
| Abstract: | "Previously, linalool was found to be the most abundant component among the cocktail of volatiles released from flowers of Freesia hybrida. Linalool formation is catalysed by monoterpene synthase TPS1. However, the regulatory network developmentally modulating the expression of the TPS1 gene in Freesia hybrida remains unexplored. In this study, three regulatory genes, FhMYB21L1, FhMYB21L2, and FhMYC2, were screened from 52 candidates. Two MYB transcription factor genes were synchronously expressed with FhTPS1 and could activate its expression significantly when overexpressed, and the binding of FhMYB21L2 to the MYBCORE sites in the FhTPS1 promoter was further confirmed, indicating a direct role in activation. FhMYC2 showed an inverse expression pattern compared with FhTPS1; its expression led to a decreased binding of FhMYB21 to the FhTPS1 promoter to reduce its activation capacity when co-expressed, suggesting a role for an MYB-bHLH complex in the regulation of the FhTPS1 gene. In Arabidopsis, both MYB21 and MYC2 regulators were shown to activate the expression of sesquiterpene synthase genes, and the regulatory roles of AtMYB21 and AtMYC2 in the expression of the linalool synthase gene were also confirmed, implying conserved functions of the MYB-bHLH complex in these two evolutionarily divergent plants. Moreover, the expression ratio between MYB21 and MYC2 orthologues might be a determinant factor in floral linalool emission" |
| Keywords: | "Alkyl and Aryl Transferases *Arabidopsis/genetics/metabolism *Arabidopsis Proteins/genetics/metabolism Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Flowers/genetics/metabolism Gene Expression Regulation, Plant Plants, Genetically Modified/m;" |
| Notes: | "MedlineYang, Zhongzhou Li, Yueqing Gao, Fengzhan Jin, Wei Li, Shuying Kimani, Shadrack Yang, Song Bao, Tingting Gao, Xiang Wang, Li eng Research Support, Non-U.S. Gov't England 2020/04/11 J Exp Bot. 2020 Jul 6; 71(14):4140-4158. doi: 10.1093/jxb/eraa184" |
