| Title: | Elucidation of the biochemical pathway of 2-phenylethanol from shikimic acid using isolated protoplasts of rose flowers |
| Author(s): | Yang Z; Sakai M; Sayama H; Shimeno T; Yamaguchi K; Watanabe N; |
| Address: | "Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan" |
| DOI: | 10.1016/j.jplph.2008.11.001 |
| ISSN/ISBN: | 1618-1328 (Electronic) 0176-1617 (Linking) |
| Abstract: | "The isolated protoplasts of rose flowers were used to investigate the metabolic pathway in rose flower leading from shikimic acid or L-phenylalanine (L-Phe) to 2-phenylethanol (2PE), a dominant volatile compound in hybrid roses such as Rosa damascena Mill., R. 'Hoh-Jun', and R. 'Yves Piaget'. Deuterium-labeled L-Phe ([2H8]L-Phe) was supplied to the protoplasts isolated from R. 'Yves Piaget' petals. The volatile end products ([2Hn]-2PE, n=6-8) and their related intermediates ([2Hn]phenylacetaldehyde, n=6-8) were detected in the protoplasts by gas chromatography-mass spectrometry (GC-MS). In addition, we chemically synthesized [2,3,4,5,6-13C5]shikimic acid, a new stable isotopomer, to investigate the formation of 2PE from shikimic acid by GC-MS and nuclear magnetic resonance. We proposed the hypothetical biochemical pathway of 2PE from shikimic acid via chorismic acid, L-Phe, and phenylacetaldehyde. This protoplast system facilitates findings of metabolic intermediates and simplifies the complex branching biosynthetic pathways of floral scents to distinct individual events" |
| Keywords: | Flowers/*metabolism *Metabolic Networks and Pathways Phenylethyl Alcohol/chemistry/*metabolism Protoplasts/*metabolism Rosa/*metabolism Shikimic Acid/chemistry/*metabolism; |
| Notes: | "MedlineYang, Ziyin Sakai, Miwa Sayama, Hironori Shimeno, Taku Yamaguchi, Koji Watanabe, Naoharu eng Research Support, Non-U.S. Gov't Germany 2008/12/23 J Plant Physiol. 2009 May 15; 166(8):887-91. doi: 10.1016/j.jplph.2008.11.001. Epub 2008 Dec 20" |
