« Previous Abstract"Iron-functionalized titanium dioxide on flexible glass fibers for photocatalysis of benzene, toluene, ethylbenzene, and o-xylene (BTEX) under visible- or ultraviolet-light irradiation"    Next Abstract[Association between volatile organic compounds and mortality risk of stroke] »

Biosci Biotechnol Biochem

Title:		Classification of fermented soybean paste during fermentation by 1H nuclear magnetic resonance spectroscopy and principal component analysis
Author(s):		Yang SO; Kim MS; Liu KH; Auh JH; Kim YS; Kwon DY; Choi HK;
Address:		"College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea"
Journal Title:		Biosci Biotechnol Biochem
Year:		2009
Volume:		20090307
Issue:		3
Page Number:		502 - 507
DOI:		10.1271/bbb.80467
ISSN/ISBN:		1347-6947 (Electronic) 0916-8451 (Linking)
Abstract:		"Fermented soybean paste (doenjang, FSP) is a traditionally fermented Korean food produced by fermentation with various microorganisms that is known to exhibit various beneficial bioactivities. To investigate the changes in nonvolatile metabolites of FSP during fermentation, samples produced with six fermentation times were analyzed using an (1)H nuclear magnetic resonance spectroscopy (NMR)-based metabolomics technique. This revealed clear separation of 50% methanol extracts of the FSP samples with different fermentation times in the principal component plots by combining PC1 and PC2, which cumulatively accounted for 94.2% of the variance. Major compounds contributing to the separation of 50% methanol extracts of FSP with various fermentation times were isoleucine/leucine, lactate, alanine, acetic acid, glutamine, choline, tyrosine, and phenylalanine. In addition, the (1)H NMR spectra of chloroform extracts were separated mainly by a combination of PC1 and PC3, which accounted for 72.6% of the variance. The present study suggests the usefulness of a (1)H NMR-based metabolomics approach to discriminate FSP samples subjected to different fermentation times, and this is the first report regarding metabolomic profiling of FSP"
Keywords:		*Fermentation Magnetic Resonance Spectroscopy Organic Chemicals/analysis/isolation & purification *Principal Component Analysis Soybeans/*chemistry/*metabolism Time Factors Volatilization Water/chemistry;
Notes:		"MedlineYang, Seung-Ok Kim, Min-Su Liu, Kwang-Hyun Auh, Joong-Hyuck Kim, Young-Suk Kwon, Dae Young Choi, Hyung-Kyoon eng Research Support, Non-U.S. Gov't England 2009/03/10 Biosci Biotechnol Biochem. 2009 Mar 23; 73(3):502-7. doi: 10.1271/bbb.80467. Epub 2009 Mar 7"