| Title: | The Key Glutathione S-Transferase Family Genes Involved in the Detoxification of Rice Gramine in Brown Planthopper Nilaparvata lugens |
| Author(s): | Yang J; Kong XD; Zhu-Salzman K; Qin QM; Cai QN; |
| Address: | "College of Plant Protection, China Agricultural University, Beijing 100193, China. MOA Key Laboratory of Crop Pest Monitoring and Green Control, College of Plant Protection, China Agricultural University, Beijing 100193, China. Department of Entomology, Texas A & M University, College Station, TX 77843, USA. College of Plant Sciences, Jilin University, Changchun 130062, China. Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Phytochemical toxins are considered a defense measure for herbivore invasion. To adapt this defensive strategy, herbivores use glutathione S-transferases (GSTs) as an important detoxification enzyme to cope with toxic compounds, but the underlying molecular basis for GST genes in this process remains unclear. Here, we investigated the basis of how GST genes in brown planthopper (BPH, Nilaparvata lugens (Stal)) participated in the detoxification of gramine by RNA interference. For BPH, the LC(25) and LC(50) concentrations of gramine were 7.11 and 14.99 mug/mL at 72 h after feeding, respectively. The transcriptions of seven of eight GST genes in BPH were induced by a low concentration of gramine, and GST activity was activated. Although interferences of seven genes reduced BPH tolerance to gramine, only the expression of NlGST1-1, NlGSTD2, and NlGSTE1 was positively correlated with GST activities, and silencing of these three genes inhibited GST activities in BPH. Our findings reveal that two new key genes, NlGSTD2 and NlGSTE1, play an essential role in the detoxification of gramine such as NlGST1-1 does in BPH, which not only provides the molecular evidence for the coevolution theory, but also provides new insight into the development of an environmentally friendly strategy for herbivore population management" |
| Keywords: | Nilaparvata lugens Oryza sativa RNA interference glutathione S-transferases gramine; |
| Notes: | "PubMed-not-MEDLINEYang, Jun Kong, Xiang-Dong Zhu-Salzman, Keyan Qin, Qing-Ming Cai, Qing-Nian eng 2016YFD0300203/the Nation Key Projects on science and technology innovation of high yield and efficiency of grain/ 2015NX001/the Chinese Universities Scientific Fund/ Switzerland 2021/12/24 Insects. 2021 Nov 25; 12(12):1055. doi: 10.3390/insects12121055" |
