| Title: | Chronic effects of benzalkonium chlorides on short chain fatty acids and methane production in semi-continuous anaerobic digestion of waste activated sludge |
| Author(s): | Yang CX; He ZW; Liu WZ; Wang AJ; Wang L; Liu J; Liu BL; Ren NQ; Yu SP; Guo ZC; |
| Address: | "Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research, School of Geography and Tourism, Harbin University, Harbin, 150086, China; National Technology Innovation Center of Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. Shanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China. State Key Laboratory of Urban Water Resource and Environment, Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China. Electronic address: liuwenzong@hit.edu.cn. State Key Laboratory of Urban Water Resource and Environment, Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China. School of Environmental & Municipal Engineering, Qingdao University of Technology, Qingdao, 266520, China. Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research, School of Geography and Tourism, Harbin University, Harbin, 150086, China. National Technology Innovation Center of Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, China" |
| DOI: | 10.1016/j.scitotenv.2022.157619 |
| ISSN/ISBN: | 1879-1026 (Electronic) 0048-9697 (Linking) |
| Abstract: | "As an emerging pollutant, benzalkonium chlorides (BACs) potentially enriched in waste activated sludge (WAS). However, the microbial response mechanism under chronic effects of BACs on acidogenesis and methanogenesis in anaerobic digestion (AD) has not been clearly disclosed. This study investigated the AD (by-)products and microbial evolution under low to high BACs concentrations from bioreactor startup to steady running. It was found that BACs can lead to an increase of WAS hydrolysis and fermentation, but a disturbance to acidogenic bacteria also occurred at low BACs concentration. A noticeable inhibition to methanogenesis occurred when BAC concentration was up to 15 mg/g TSS. Metagenomic analysis revealed the key genes involved in acetic acid (HAc) biosynthesis (i.e. phosphate acetyltransferase, PTA), beta-oxidation pathway (acetyl-CoA C-acetyltransferase) and propionic acid (HPr) conversion was slightly promoted compared with control. Furthermore, BACs inhibited the acetotrophic methanogenesis (i.e. acetyl-CoA synthetase), especially BAC concentration was up to 15 mg/g TSS, thereby enhanced short chain fatty acids (SCFAs) accumulation. Overall, chronic stimulation of functional microorganisms with increasing concentrations of BACs impact WAS fermentation" |
| Keywords: | "Acetyl Coenzyme A/metabolism Acetyl-CoA C-Acetyltransferase/metabolism Anaerobiosis Benzalkonium Compounds Bioreactors/microbiology *Environmental Pollutants Fatty Acids, Volatile/metabolism Fermentation Ligases/metabolism Methane Phosphate Acetyltransfer;" |
| Notes: | "MedlineYang, Chun-Xue He, Zhang-Wei Liu, Wen-Zong Wang, Ai-Jie Wang, Ling Liu, Jia Liu, Bao-Ling Ren, Nan-Qi Yu, Shao-Peng Guo, Ze-Chong eng Netherlands 2022/07/29 Sci Total Environ. 2022 Nov 15; 847:157619. doi: 10.1016/j.scitotenv.2022.157619. Epub 2022 Jul 25" |
