| Title: | Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum) |
| Author(s): | Yang CQ; Wu XM; Ruan JX; Hu WL; Mao YB; Chen XY; Wang LJ; |
| Address: | "National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China" |
| DOI: | 10.1016/j.phytochem.2013.09.009 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "Cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. However, to date little is known about the biosynthesis of volatile terpenes in this crop. Herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, Gossypium hirsutum cv. CCRI12, were detected by gas chromatography-mass spectrometry (GC-MS). By EST data mining combined with Rapid Amplification of cDNA Ends (RACE), full-length cDNAs of three terpene synthases (TPSs), GhTPS1, GhTPS2 and GhTPS3 were isolated. By in vitro assays of the recombinant proteins, it was found that GhTPS1 and GhTPS2 are sesquiterpene synthases: the former converted farnesyl pyrophosphate (FPP) into beta-caryophyllene and alpha-humulene in a ratio of 2:1, whereas the latter produced several sesquiterpenes with guaia-1(10),11-diene as the major product. By contrast, GhTPS3 is a monoterpene synthase, which produced alpha-pinene, beta-pinene, beta-phellandrene and trace amounts of other monoterpenes from geranyl pyrophosphate (GPP). The TPS activities were also supported by Virus Induced Gene Silencing (VIGS) in the cotton plant. GhTPS1 and GhTPS3 were highly expressed in the cotton plant overall, whereas GhTPS2 was expressed only in leaves. When stimulated by mechanical wounding, Verticillium dahliae (Vde) elicitor or methyl jasmonate (MeJA), production of terpenes and expression of the corresponding synthase genes were induced. These data demonstrate that the three genes account for the biosynthesis of volatile terpenes of cotton, at least of this Upland cotton" |
| Keywords: | Acetates/metabolism Alkyl and Aryl Transferases/*isolation & purification/*metabolism Bicyclic Monoterpenes Bridged Bicyclo Compounds/metabolism Cyclohexane Monoterpenes Cyclohexenes/metabolism Cyclopentanes/metabolism Gas Chromatography-Mass Spectrometry; |
| Notes: | "MedlineYang, Chang-Qing Wu, Xiu-Ming Ruan, Ju-Xin Hu, Wen-Li Mao, Yin-Bo Chen, Xiao-Ya Wang, Ling-Jian eng Research Support, Non-U.S. Gov't England 2013/10/01 Phytochemistry. 2013 Dec; 96:46-56. doi: 10.1016/j.phytochem.2013.09.009. Epub 2013 Sep 24" |
