| Title: | Functional characterization of the eugenol synthase gene (RcEGS1) in rose |
| Author(s): | Yan H; Baudino S; Caissard JC; Florence N; Zhang H; Tang K; Li S; Lu S; |
| Address: | "School of Life Sciences, Yunnan University, Kunming, 650091, PR China; Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, PR China. Univ Lyon, UJM-Saint-Etienne, CNRS, BVpam FRE 3727, F-42023, Saint-Etienne, France. Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, PR China. School of Life Sciences, Yunnan University, Kunming, 650091, PR China. Electronic address: shuganglu@163.com" |
| DOI: | 10.1016/j.plaphy.2018.05.015 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "The floral volatile compound eugenol is an important constituent in many aromatic plants, being a floral attractant for pollinators as well as having antimicrobial activity. Rose flowers emit eugenol and its derivatives. We recently reported a eugenol synthase gene (RcEGS1) (JQ522949) that was present in petals of R. chinensis cv. Old Blush. RcEGS1 has its highest expression levels in the petals compared to other tissues; it has higher transcript levels at the developmental blooming stage and lower levels at budding and senescence stages. Here, we overexpressed the RcEGS1 protein in Escherichia coli, and showed by Western-blot analysis that its expression was mainly detected in stamens and petals at the flower opening stage. RcEGS1 was principally localized in the upper and lower epidermal layers, which are the major sites of scent emission in roses. Furthermore, we demonstrated that down-regulation of RcEGS1 expression in flowers by virus-induced gene silencing led to a reduction of the relative content of eugenol. We suggested that RcEGS1 was responsible for eugenol biosynthesis in roses" |
| Keywords: | "Blotting, Western Down-Regulation Escherichia coli Eugenol/*metabolism Flowers/metabolism Gene Expression Regulation, Plant Genes, Plant/*genetics/physiology Microorganisms, Genetically-Modified Plant Proteins/*genetics/physiology Rosa/enzymology/*genetic;" |
| Notes: | "MedlineYan, Huijun Baudino, Sylvie Caissard, Jean-Claude Florence Nicole Zhang, Hao Tang, Kaixue Li, Shubin Lu, Shugang eng France 2018/05/23 Plant Physiol Biochem. 2018 Aug; 129:21-26. doi: 10.1016/j.plaphy.2018.05.015. Epub 2018 May 10" |
