« Previous AbstractBacteria isolated from Bengal cat (Felis catus x Prionailurus bengalensis) anal sac secretions produce volatile compounds potentially associated with animal signaling    Next AbstractIncreases in Fos-immunoreactivity after exposure to a combination of two male urinary components in the accessory olfactory bulb of the female rat »

J Breath Res

Title:		Modeling cellular metabolomic effects of oxidative stress impacts from hydrogen peroxide and cigarette smoke on human lung epithelial cells
Author(s):		Yamaguchi MS; McCartney MM; Falcon AK; Linderholm AL; Ebeler SE; Kenyon NJ; Harper RH; Schivo M; Davis CE;
Address:		"Mechanical and Aerospace Engineering, University of California Davis, Davis, CA 95616, United States of America. Center for Comparative Respiratory Biology and Medicine, UC Davis Medical School, Davis, CA 95616, United States of America. Viticulture and Enology, University of California Davis, One Shields Avenue, Davis, CA 95616, United States of America. Department of Internal Medicine, 4150 V Street, Suite 3400, University of California, Davis, Sacramento, CA 95817, United States of America. VA Northern California Health Care System, 10535 Hospital Way, Mather, CA 95655, United States of America"
Journal Title:		J Breath Res
Year:		2019
Volume:		20190619
Issue:		3
Page Number:		36014 -
DOI:		10.1088/1752-7163/ab1fc4
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Print) 1752-7155 (Linking)
Abstract:		"The respiratory system is continuously exposed to variety of biological and chemical irritants that contain reactive oxygen species, and these are well known to cause oxidative stress responses in lung epithelial cells. There is a clinical need to identify biomarkers of oxidative stress which could potentially support early indicators of disease and health management. To identify volatile biomarkers of oxidative stress, we analyzed the headspace above human bronchial epithelial cell cultures (HBE1) before and after hydrogen peroxide (H(2)O(2)) and cigarette smoke extract (CSE) exposure. Using stir bar and headspace sorptive extraction-gas chromatography-mass spectrometry, we searched for volatile organic compounds (VOC) of these oxidative measures. In the H(2)O(2) cell peroxidation experiments, four different H(2)O(2) concentrations (0.1, 0.5, 10, 50 mM) were applied to the HBE1 cells, and VOCs were collected every 12 h over the time course of 48 h. In the CSE cell peroxidation experiments, four different smoke extract concentrations (0%, 10%, 30%, 60%) were applied to the cells, and VOCs were collected every 12 h over the time course of 48 h. We used partial-least squares (PLS) analysis to identify putative compounds from the mass spectrometry results that highly correlated with the known applied oxidative stress. We observed chemical emissions from the cells that related to both the intensity of the oxidative stress and followed distinct time courses. Additionally, some of these chemicals are aldehydes, which are thought to be non-invasive indicators of oxidative stress in exhaled human breath. Together, these results illustrate a powerful in situ cell culture model of oxidative stress that can be used to explore the putative biological genesis of exhaled breath biomarkers that are often observed in human clinical studies"
Keywords:		"Cell Line Cell Survival/drug effects Epithelial Cells/drug effects/*pathology Humans Hydrogen Peroxide/*toxicity Least-Squares Analysis Lung/*pathology Metabolomics/*methods *Models, Biological *Oxidative Stress/drug effects Smoking/*adverse effects Volat;"
Notes:		"MedlineYamaguchi, Mei S McCartney, Mitchell M Falcon, Alexandria K Linderholm, Angela L Ebeler, Susan E Kenyon, Nicholas J Harper, Richart H Schivo, Michael Davis, Cristina E eng U01 EB022003/EB/NIBIB NIH HHS/ T32 HL007013/HL/NHLBI NIH HHS/ P30 ES023513/ES/NIEHS NIH HHS/ UL1 TR000002/TR/NCATS NIH HHS/ UG3 OD023365/OD/NIH HHS/ K23 HL127185/HL/NHLBI NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2019/05/08 J Breath Res. 2019 Jun 19; 13(3):036014. doi: 10.1088/1752-7163/ab1fc4"