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Environ Microbiol Rep

Title:		"Elucidating the interaction of carbon, nitrogen, and temperature on the biosynthesis of Aureobasidium pullulans antifungal volatiles"
Author(s):		Yalage Don SM; Gambetta JM; Steel CC; Schmidtke LM;
Address:		"School of Agricultural and Wine Sciences, National Wine and Grape Industry Centre, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia. South Australian Research and Development Institute Waite Campus, GPO Box 397, Adelaide, SA, 5001, Australia"
Journal Title:		Environ Microbiol Rep
Year:		2021
Volume:		20210114
Issue:		4
Page Number:		482 - 494
DOI:		10.1111/1758-2229.12925
ISSN/ISBN:		1758-2229 (Electronic) 1758-2229 (Linking)
Abstract:		"The combined biochemical impact of carbon, nitrogen and temperature on the biosynthesis of the antifungal volatile organic compounds (VOCs): ethanol, 2-methyl-1-propanol, 3-methyl-1-butanol and 2-phenylethanol produced by Aureobasidium pullulans A1 and A3 was investigated using a Box-Behnken experimental design and response surface methodology (RSM). Normalized peak areas derived from solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis, indicated that initial carbon content had a significant influence on the biosynthesis of ethanol and alcohols with greater than three carbon atoms. This result suggests a dominant activity of the A. pullulans anabolic pathway to biosynthesize three higher alcohols via de novo biosynthesis of amino acids from sugar metabolism. Low concentrations of carbon (3-13 g l(-1) ) with nitrogen as both ammonium and amino acids in the growth medium resulted in a higher number of significant linear and quadratic relationships. Nitrogen availability and growth temperature had significant negative linear and quadratic correlations with VOCs biosynthesis in most instances. Isolate-dependant metabolic response was evident for all abiotic parameters tested on alcohol production. The findings of this study offer new perspectives to improve the production of key antifungal compounds by antagonists in biological control systems"
Keywords:		*Antifungal Agents/pharmacology Aureobasidium Carbon *Nitrogen/metabolism Temperature;
Notes:		"MedlineYalage Don, Sashika M Gambetta, Joanna M Steel, Christopher C Schmidtke, Leigh M eng Research Support, Non-U.S. Gov't 2021/01/16 Environ Microbiol Rep. 2021 Aug; 13(4):482-494. doi: 10.1111/1758-2229.12925. Epub 2021 Jan 14"