| Title: | "Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans" |
| Author(s): | Xu X; Wang Y; Bi H; Xu J; Liu Z; Niu C; He L; James AA; Li K; Huang Y; |
| Address: | "School of Life Science, East China Normal University, 200241, Shanghai, China; Key Laboratory of Insect Developmental and Evolutionary Biology, Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 200032, Shanghai, China. Key Laboratory of Insect Developmental and Evolutionary Biology, Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 200032, Shanghai, China; Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science & Technology, Huazhong Agricultural University, 430070, Wuhan, China. Key Laboratory of Insect Developmental and Evolutionary Biology, Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 200032, Shanghai, China. Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science & Technology, Huazhong Agricultural University, 430070, Wuhan, China. School of Life Science, East China Normal University, 200241, Shanghai, China. Electronic address: lhe@bio.ecnu.edu.cn. Department of Microbiology & Molecular Genetics and Molecular Biology & Biochemistry, University of California, Irvine, CA, 92697-3900, USA. School of Life Science, East China Normal University, 200241, Shanghai, China. Electronic address: kaili@bio.ecnu.edu.cn. Key Laboratory of Insect Developmental and Evolutionary Biology, Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 200032, Shanghai, China. Electronic address: yphuang@sibs.ac.cn" |
| DOI: | 10.1016/j.ibmb.2019.103243 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Sterile insect technology (SIT) is an environmentally friendly method for pest control. As part of our efforts to develop a strategy that results in engineered male-sterile strains with minimum effects on viability and mating competition, we used CRISPR/Cas9 technology to disrupt Ser2, which encodes a seminal fluid protein, in the model lepidopteran insect, Bombyx mori, and an important agricultural pest, Plutella xylostella. Disruption of Ser2 resulted in dominant heritable male sterility. Wild-type females mated with Ser2-deficient males laid eggs normally, but the eggs did not hatch. We detected no differences in other reproductive behaviors in the mutant males. These results support the conclusion that Ser2 gene is necessary for male reproductive success in diverse lepidopterans. Targeting Ser2 gene has the potential to form the basis for a new strategy for pest control" |
| Keywords: | "Animals Bombyx/genetics/physiology CRISPR-Cas Systems Infertility, Male/genetics Insect Proteins/*genetics/metabolism Male Moths/genetics/*physiology Mutation Reproduction/genetics Serine Proteases/*genetics/metabolism Bombyx mori CRISPR/Cas9 Male sterili;" |
| Notes: | "MedlineXu, Xia Wang, Yaohui Bi, Honglun Xu, Jun Liu, Zulian Niu, Changying He, Lin James, Anthony A Li, Kai Huang, Yongping eng Research Support, Non-U.S. Gov't England 2019/09/22 Insect Biochem Mol Biol. 2020 Jan; 116:103243. doi: 10.1016/j.ibmb.2019.103243. Epub 2019 Sep 18" |
