| Title: | Chemosensory receptor genes in the Oriental tobacco budworm Helicoverpa assulta |
| Author(s): | Xu W; Papanicolaou A; Liu NY; Dong SL; Anderson A; |
| Address: | "CSIRO Ecosystem Sciences, Canberra, ACT, Australia" |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "The Oriental tobacco budworm (Helicoverpa assulta) is a specialist herbivore moth and its larvae feed on Solanaceous plants. (Z)-9-hexadecenal (Z9-16: Ald) is the major sex pheromone component in H. assulta but the specific pheromone receptor (PR) against Z9-16: Ald has not yet been identified. In the present study, we integrated transcriptomic, bioinformatic and functional characterization approaches to investigate the chemosensory receptor genes of H. assulta. We identified seven potential PRs with 44 olfactory receptors, 18 gustatory receptors and 24 ionotropic receptors, which were further studied by in silico gene expression profile, phylogenetic analysis, reverse transcription PCR and calcium imaging assays. The candidate PR, HassOR13, showed a strong response to the minor sex pheromone component, (Z)-11-hexadecenal, but not the major component, Z9-16: Ald, in calcium imaging assays. This study provides the molecular basis for comparative studies of chemosensory receptors between H. assulta and other Helicoverpa species and will advance our understanding of the evolution and function of Lepidoptera insect chemosensation" |
| Keywords: | "Aldehydes/metabolism Animals Calcium/metabolism Computer Simulation Gene Expression Profiling Larva/genetics Molecular Sequence Data Moths/*genetics/metabolism Phylogeny Receptors, Odorant/genetics/metabolism Receptors, Pheromone/*genetics/metabolism Reve;" |
| Notes: | "MedlineXu, W Papanicolaou, A Liu, N-Y Dong, S-L Anderson, A eng Research Support, Non-U.S. Gov't England 2014/11/29 Insect Mol Biol. 2015 Apr; 24(2):253-63. doi: 10.1111/imb.12153. Epub 2014 Nov 27" |
