« Previous AbstractGut-Associated Bacteria of Dendroctonus valens and their Involvement in Verbenone Production    Next AbstractComparative study of volatile components from male and female flower buds of Populus x tomentosa by HS-SPME-GC-MS »

Insect Mol Biol

Title:		Calmodulin as a downstream gene of octopamine-OAR alpha1 signalling mediates olfactory attraction in gregarious locusts
Author(s):		Xu L; Li L; Yang P; Ma Z;
Address:		"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China"
Journal Title:		Insect Mol Biol
Year:		2017
Volume:		20161007
Issue:		1
Page Number:		1 - 12
DOI:		10.1111/imb.12266
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"The migratory locust (Locusta migratoria) shows aggregative traits in nymph marching bands and swarm formations through mutual olfactory attraction of conspecifics. However, olfactory preference in different nymph stages in gregarious locusts is not sufficiently explored. In this study, we found that the nymph olfactory preference for gregarious volatiles exhibited obvious variations at different developmental stages. The gregarious locusts show attractive response to conspecific volatiles from the third stadium. Transcriptome comparison between third- and fourth-stadium nymphs showed that the G protein-coupled receptor (GPCR) pathways are significantly enriched. Amongst the genes present in GPCR pathways, the expression level of calmodulin in locust brains significantly increased from the third- to the fourth-stadium nymphs. Amongst the four octopamine receptors (OARs) belonging to the GPCR family, only OAR alpha1 showed similar expression patterns to those of calmodulin, and knockdown of OAR alpha1 reduced the expression level of calmodulin. RNA interference of calmodulin decreased locomotion and induced the loss of olfactory attraction in gregarious locusts. Moreover, the activation of OAR alpha1 in calmodulin-knockdown locusts did not induce olfactory attraction of the nymphs to gregarious volatiles. Thus, calmodulin as a downstream gene of octopamine-OAR alpha1 (OA-OAR alpha1) signalling mediates olfactory attraction in gregarious locusts. Overall, this study provides novel insights into the mechanism of OA-OAR alpha1 signalling involved in olfactory attraction of gregarious locusts"
Keywords:		"Animals Brain/metabolism Calmodulin/*metabolism Insect Proteins/metabolism Locusta migratoria/genetics/growth & development/*metabolism Nymph/metabolism *Pheromones Receptors, Biogenic Amine/*metabolism *Smell OAR alpha1 aggregation calmodulin gregarious;"
Notes:		"MedlineXu, L Li, L Yang, P Ma, Z eng Research Support, Non-U.S. Gov't England 2016/10/08 Insect Mol Biol. 2017 Feb; 26(1):1-12. doi: 10.1111/imb.12266. Epub 2016 Oct 7"