« Previous AbstractEffect of mechanical vibration on postharvest quality and volatile compounds of blueberry fruit    Next AbstractInhibition of Ty1 transposition by mating pheromones in Saccharomyces cerevisiae »

Pest Manag Sci

Title:		"Metabolic engineering of a 1,8-cineole synthase enhances aphid repellence and increases trichome density in transgenic tobacco (Nicotiana tabacum L.)"
Author(s):		Xu G; Zheng Q; Wei P; Zhang J; Liu P; Zhang H; Zhai N; Li X; Xu X; Chen Q; Cao P; Zhao J; Zhou H;
Address:		"China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, P.R. China. Tobacco Research Center, Technology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, P.R. China. National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, P.R. China"
Journal Title:		Pest Manag Sci
Year:		2023
Volume:		20230517
Issue:		9
Page Number:		3342 - 3353
DOI:		10.1002/ps.7520
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: The green peach aphid (Myzus persicae Sulzer) is a harmful agricultural pest that causes severe crop damage by directly feeding or indirectly vectoring viruses. 1,8-cineole synthase (CINS) is a multiproduct enzyme that synthesizes monoterpenes, with 1,8-cineole dominating the volatile organic compound profile. However, the relationship between aphid preference and CINS remains elusive. RESULTS: Here, we present evidence that SoCINS, a protein from garden sage (Salvia officinalis), enhanced aphid repellence and increased trichome density in transgenic tobacco. Our results demonstrated that overexpression of SoCINS (SoCINS-OE) led to the emission of 1,8-cineole at a level of up to 181.5 ng per g fresh leaf. Subcellular localization assay showed that SoCINS localized to chloroplasts. A Y-tube olfactometer assay and free-choice assays revealed that SoCINS-OE plants had a repellent effect on aphids, without incurring developmental or fecundity-related penalties. Intriguingly, the SoCINS-OE plants displayed an altered trichome morphology, showing increases in trichome density and in the relative proportion of glandular trichomes, as well as enlarged glandular cells. We also found that SoCINS-OE plants had significantly higher jasmonic acid (JA) levels than wild-type plants. Furthermore, application of 1,8-cineole elicited increased JA content and trichome density. CONCLUSION: Our results demonstrate that SoCINS-OE plants have a repellent effect on aphids, and suggest an apparent link between 1,8-cineole, JA and trichome density. This study presents a viable and sustainable approach for aphid management by engineering the expression of 1,8-cineole synthase gene in plants, and underscores the potential usefulness of monoterpene synthase for pest control. (c) 2023 Society of Chemical Industry"
Keywords:		"1, 8-cineole synthase aphid repellence monoterpene tobacco trichome density;"
Notes:		"PublisherXu, Guoyun Zheng, Qingxia Wei, Pan Zhang, Jianfeng Liu, Pingping Zhang, Hui Zhai, Niu Li, Xiaoxu Xu, Xiangli Chen, Qiansi Cao, Peijian Zhao, Jian Zhou, Huina eng Key Scientific and Technological Project of Henan Province/ National Natural Science Foundation of China/ Project of Tobacco Genome/ England 2023/05/03 Pest Manag Sci. 2023 Sep; 79(9):3342-3353. doi: 10.1002/ps.7520. Epub 2023 May 17"