| Title: | Liver Receptor homolog-1 Regulates Apoptosis of Bovine Ovarian Granulosa Cells by Progestogen Receptor Signaling Pathway |
| Author(s): | Xu D; Jiang X; Wang Y; Song S; |
| Address: | "Chongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing 400715, China. College of Animal Science and Technology, Northwest A&F University, No. 3 Taicheng Road, Xianyang 712100, China" |
| ISSN/ISBN: | 2076-2615 (Print) 2076-2615 (Electronic) 2076-2615 (Linking) |
| Abstract: | "The purpose of the present investigation was to assess the function of LRH-1 on GCs and the mechanisms involved. Here, LRH- was highly expressed in the bovine GCs of atretic follicles. Treatment with 50 muM of LRH-1 agonist (DLPC) significantly induced the expression of LRH-1 (p < 0.05). In particular, LRH-1 activation blocked the progestogen receptor signaling pathway via downregulating progesterone production and progestogen receptor levels (p < 0.05), but had no effect on 17 beta-estradiol synthesis. Meanwhile, LRH-1 activation promoted the apoptosis of GCs and increased the activity of caspase 3 (p < 0.05). Importantly, upregulating the progestogen receptor signaling pathway with progestogen could attenuate the LRH-1-induced proapoptotic effect. Moreover, treatment with progestogen decreased the activity of the proapoptotic gene caspase 3 and increased the expression of antiapoptotic gene Bcl2 in LRH-1 activated GCs (p < 0.05). Taken together, these results demonstrate that LRH-1 might be dependent on the progestogen receptor signaling pathway to modulate bovine follicular atresia" |
| Keywords: | Lrh-1 apoptosis granulosa cells progestogen; |
| Notes: | "PubMed-not-MEDLINEXu, Dejun Jiang, Xiaohan Wang, Yukun Song, Shuaifei eng 32102623/National Natural Science Foundation of China/ cstc2021jcyj-msxmX0875/Natural Science Foundation of Chongqing/ Switzerland 2022/05/15 Animals (Basel). 2022 May 8; 12(9):1213. doi: 10.3390/ani12091213" |
