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Comp Biochem Physiol Part D Genomics Proteomics

Title:		Identification and analysis of olfactory genes in Dioryctria abietella based on the antennal transcriptome
Author(s):		Xing Y; Thanasirungkul W; Adeel MM; Yu J; Aslam A; Chi DF;
Address:		"Key Laboratory for Sustainable Forest Ecosysttem Management of Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China. Agricultural Bioinformatics Key Laboratory of Hubei Province, Hubei Engineering Technology Research Center of Agricultural Big Data, College of Informatics, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China. Key Laboratory for Sustainable Forest Ecosysttem Management of Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China. Electronic address: chidefunefu@126.com"
Journal Title:		Comp Biochem Physiol Part D Genomics Proteomics
Year:		2021
Volume:		20210215
Issue:
Page Number:		100814 -
DOI:		10.1016/j.cbd.2021.100814
ISSN/ISBN:		1878-0407 (Electronic) 1744-117X (Linking)
Abstract:		"The coneworm Dioryctria abietella (Lepidoptera: Pyralidae) is an economy devastating pest that infests many valuable conifer species in the Holarctic regions, such as Pinus koraiensis Siebold and Zucc. The chemosensory system plays a crucial role in the mating, foraging, and ovipositing of this pest, and therefore it is desirable to identify chemosensory molecules for pest control. However, little is known at molecular level about the olfactory mechanisms in D. abietella. In the present study, we first established antennal transcriptomes of D. abietella and identified 132 putative chemosensory genes, including 15 odorant-binding proteins, 18 chemosensory proteins, 65 odorant receptors, 5 sensory neuron membrane proteins, 24 ionotropic receptors, and 5 gustatory receptors. In addition, phylogenetic trees were constructed for chemosensory genes to investigate the orthologs between D. abietella and other species of insects. Furthermore, we also compared the patterns of motifs between OBPs and CSPs using MEME. Additionally, we observed that most of DabiOBPs and DabiCSPs had the antenna-biased expression by quantitative real-time PCR (RT-qPCR), and there was a higher expression of DabiPBP1 and DabiPBP2 in male antennae than in female antennae. The binding sites of DabiPBPs (DabiPBP1, DabiPBP2) and DabiPRs (DabiOR19, DabiOR31) to the sex pheromone were predicted well by three-dimensional docking structure modelling and molecular docking. Our finding supplied a foundation for further research on the binding process of OBPs or CSPs and sensing process of ORs, SNMPs, IRs or GRs in D. abietella"
Keywords:		"Animals Arthropod Antennae/*physiology Female Gene Expression Profiling *Genes, Insect Insect Proteins/genetics Lepidoptera/*genetics/*physiology Male Phylogeny Receptors, Odorant/genetics Smell Transcriptome Chemosensory genes Dioryctria abietella Odoran;"
Notes:		"MedlineXing, Ya Thanasirungkul, Wariya Adeel, Muhammad Muzammal Yu, Jia Aslam, Asad Chi, De-Fu eng Research Support, Non-U.S. Gov't Netherlands 2021/03/12 Comp Biochem Physiol Part D Genomics Proteomics. 2021 Jun; 38:100814. doi: 10.1016/j.cbd.2021.100814. Epub 2021 Feb 15"