« Previous Abstractbeta-Sitosterol Attenuates High Grain Diet-Induced Inflammatory Stress and Modifies Rumen Fermentation and Microbiota in Sheep    Next AbstractEffect of Cold Stabilization Duration on Organic Acids and Aroma Compounds during Vitis vinifera L. cv. Riesling Wine Bottle Storage »

Neuroscience

Title:		Epigenetic modification of vomeronasal (V2r) precursor neurons by histone deacetylation
Author(s):		Xia J; Broad KD; Emson PC; Keverne EB;
Address:		"Babraham Institute, Babraham, Cambridge CB22 4AT, UK"
Journal Title:		Neuroscience
Year:		2010
Volume:		20100608
Issue:		3
Page Number:		1462 - 1472
DOI:		10.1016/j.neuroscience.2010.05.071
ISSN/ISBN:		1873-7544 (Electronic) 0306-4522 (Linking)
Abstract:		"Vomeronasal neurons undergo continuous neurogenesis throughout development and adult life. These neurons originate as stem cells in the apical zone of the lumen of the vomeronasal organ (VNO) and are described as nestin-expressing glia-like progenitor cells (Murdoch and Roskams, 2008). They then migrate horizontally along the basal zone where they differentiate into functional VNO neurons (Kaba et al., 1988). We harvested progenitor cells from the adult VNO and, after 3-6 months of invitro culture, these VNO neurons remained in a stable undifferentiated state expressing nestin, beta-tubulin III and vomeronasal type 2 (V2r), but not vomeronasal type 1 (V1r) receptors. Application of histone-deacetylase inhibitors induced development of a neural phenotype that expressed V2r receptors, a down-regulation of nestin expression and no change in any specific genetic markers associated with glial cells. Treatment with valproic acid induced extensive changes in gene expression in the axon guidance pathway. The adult VNO is known to functionally adapt throughout life as a consequence of changes in both a mouse's physiological status and its social environment. These pluripotent cultured neurons may provide valuable insights into how changes in both physiology and environment, exert epigenetic effects on vomeronasal neurons as they undergo continuous neurogenesis and development throughout the life of a mouse"
Keywords:		"Animals Cell Differentiation Cells, Cultured *Epigenesis, Genetic Female Gene Expression Profiling Genetic Markers Histone Deacetylase Inhibitors/*pharmacology Histone Deacetylases/*metabolism Hydroxamic Acids/pharmacology Mice Microtubule-Associated Prot;neuroscience;"
Notes:		"MedlineXia, J Broad, K D Emson, P C Keverne, E B eng 2010/07/03 Neuroscience. 2010 Sep 1; 169(3):1462-72. doi: 10.1016/j.neuroscience.2010.05.071. Epub 2010 Jun 8"