| Title: | 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage induced by environmental tobacco side-stream smoke and its mechanism |
| Author(s): | Xi ZG; Chao FH; Yang DF; Zhang HS; Zhang W; |
| Address: | "Institute of Hygiene and Environmental Medicine, Tianjin 300050, China" |
| ISSN/ISBN: | 0895-3988 (Print) 0895-3988 (Linking) |
| Abstract: | "OBJECTIVE: To study the genotoxicity effect of environmental tobacco side-stream smokes (ETSS) on oxidative DNA damage and its molecular mechanism. METHODS: DNA adduct 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. The level of 8-OHdG in DNA exposed to ETSS was detected by high performance liquid chromatography with electrochemical detection. Organic and inorganic components in ETSS were analyzed by gas chromatography-mass spectrum and atomic absorption spectrum respectively. RESULTS: Particle matters (PMs) and volatile organic compounds (VOCs) in ETSS could directly induce oxidative DNA damage and formation of 8-OHdG. There were 123 and 84 kinds of organic components in PMs and VOCs respectively, and 7 kinds of inorganic components in ETSS. Some components, especially quinones and polyphenols in ETSS, could produce free radicals in vitro by auto-oxidation without any biological activity systems, and with the catalytic reaction of metals, the DNA adduct 8-OHdG was produced. CONCLUSION: ETSS have biological oxidative effect on DNA in vitro and in vivo, and expressed direct genotoxicity. 8-OHdG is a valuable biomarker of oxidative DNA damage" |
| Keywords: | "8-Hydroxy-2'-Deoxyguanosine Animals Biomarkers/analysis Cattle DNA/drug effects/metabolism DNA Adducts/*analysis *DNA Damage Deoxyguanosine/*analogs & derivatives/analysis Female Lung/chemistry/metabolism Metals, Heavy/analysis Organic Chemicals/analysis;" |
| Notes: | "MedlineXi, Zhu-Ge Chao, Fu-Huan Yang, Dan-Feng Zhang, Hua-Shan Zhang, Wei eng Research Support, Non-U.S. Gov't China 2005/05/03 Biomed Environ Sci. 2005 Feb; 18(1):43-7" |
