| Title: | Alpha-agglutinin expression in Saccharomyces cerevisiae |
| Author(s): | Wojciechowicz D; Lipke PN; |
| Address: | "Department of Biological Sciences, Hunter College, New York 10021" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/0006-291x(89)91557-x |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the antibody, we have determined that 1) the surface distribution of alpha-agglutinin on alpha cells is polar, 2) about 5 x 10(4) molecules/cell are constitutively expressed on strain X2180-1B (alpha) cells, and 3) treatment of alpha cells with the sex pheromone a-factor causes an increase in cell surface alpha-agglutinin, consistent with the a-factor induced increase in cell agglutinability" |
| Keywords: | "Agglutinins/*analysis/immunology Antibodies, Fungal Antibody Specificity Antigen-Antibody Reactions Fungal Proteins/*analysis/immunology Mating Factor Membrane Glycoproteins/analysis/immunology Peptides/*analysis/immunology Saccharomyces cerevisiae/*analy;" |
| Notes: | "MedlineWojciechowicz, D Lipke, P N eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1989/05/30 Biochem Biophys Res Commun. 1989 May 30; 161(1):46-51. doi: 10.1016/0006-291x(89)91557-x" |
