| Title: | A metabolomic study of vegetative incompatibility in Cryphonectria parasitica |
| Author(s): | Witte TE; Shields S; Heberlig GW; Darnowski MG; Belov A; Sproule A; Boddy CN; Overy DP; Smith ML; |
| Address: | "Carleton University, Department of Biology, Ottawa, Canada. Electronic address: tom.witte@agr.gc.ca. Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Canada. Electronic address: samuel.shields@austin.utexas.edu. University of Ottawa, Department of Chemistry and Biomolecular Sciences, Ottawa, Canada. Electronic address: gwheberlig@ucsd.edu. University of Ottawa, Department of Chemistry and Biomolecular Sciences, Ottawa, Canada. Electronic address: mdarn096@uottawa.ca. Carleton University, Department of Biology, Ottawa, Canada. Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Canada. Electronic address: amanda.sproule@agr.gc.ca. University of Ottawa, Department of Chemistry and Biomolecular Sciences, Ottawa, Canada. Electronic address: cboddy@uottawa.ca. Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Canada. Electronic address: david.overy@agr.gc.ca. Carleton University, Department of Biology, Ottawa, Canada. Electronic address: MyronSmith@cunet.carleton.ca" |
| DOI: | 10.1016/j.fgb.2021.103633 |
| ISSN/ISBN: | 1096-0937 (Electronic) 1087-1845 (Linking) |
| Abstract: | "Vegetative incompatibility (VI) is a form of non-self allorecognition in filamentous fungi that restricts conspecific hyphal fusion and the formation of heterokaryons. In the chestnut pathogenic fungus, Cryphonectria parasitica, VI is controlled by six vic loci and has been of particular interest because it impedes the spread of hypoviruses and thus biocontrol strategies. We use nuclear magnetic resonance and high-resolution mass spectrometry to characterize alterations in the metabolome of C. parasitica over an eight-day time course of vic3 incompatibility. Our findings support transcriptomic data that indicated remodeling of secondary metabolite profiles occurs during vic3 -associated VI. VI-associated secondary metabolites include novel forms of calbistrin, decumbenone B, a sulfoxygenated farnesyl S-cysteine analog, lysophosphatidylcholines, and an as-yet unidentified group of lipid disaccharides. The farnesyl S-cysteine analog is structurally similar to pheromones predicted to be produced during VI and is here named 'crypheromonin'. Mass features associated with C. parasitica secondary metabolites skyrin, rugulosin and cryphonectric acid were also detected but were not VI specific. Partitioning of VI-associated secondary metabolites was observed, with crypheromonins and most calbistrins accumulating in the growth medium over time, whereas lysophosphatidylcholines, lipid disaccharide-associated mass features and other calbistrin-associated mass features peaked at distinct time points in the mycelium. Secondary metabolite biosynthetic gene clusters and potential biological roles associated with the detected secondary metabolites are discussed" |
| Keywords: | *Ascomycota/genetics Metabolomics Mycelium *RNA Viruses Calbistrin Cryphonectria parasitica Secondary metabolite biosynthetic gene clusters Vegetative incompatibility; |
| Notes: | "MedlineWitte, Thomas E Shields, Sam Heberlig, Graham W Darnowski, Mike G Belov, Anatoly Sproule, Amanda Boddy, Christopher N Overy, David P Smith, Myron L eng Research Support, Non-U.S. Gov't 2021/10/08 Fungal Genet Biol. 2021 Dec; 157:103633. doi: 10.1016/j.fgb.2021.103633. Epub 2021 Oct 5" |
