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J Biol Chem

Title:		"Isolation and characterization of a novel, developmentally regulated proteinase inhibitor I protein and cDNA from the fruit of a wild species of tomato"
Author(s):		Wingate VP; Broadway RM; Ryan CA;
Address:		"Institute of Biological Chemistry, Washington State University, Pullman 99164-6340"
Journal Title:		J Biol Chem
Year:		1989
Volume:		264
Issue:		30
Page Number:		17734 - 17738
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"A novel member of the proteinase Inhibitor I family having a trypsin inhibitor specificity was isolated from the fruit of the wild tomato species Lycopersicon peruvianum (L.) Mill. (LA 107) and characterized. The protein is among the isoinhibitors of Inhibitor I that comprise 50% of the soluble proteins in the fruit of this wild species of tomato. A cDNA corresponding to the inhibitor protein and mRNA was isolated and characterized. The Inhibitor I mRNA represented 0.06% of the poly(A) RNA and gene copy number reconstruction experiments gave an estimate of two to four genes/haploid genome. The open reading frame of the cDNA codes for a protein of 111 amino acids having a 42-amino acid prepropolypeptide. The NH2-terminal sequence of the first 21 amino acids of the purified Inhibitor I protein confirmed that the cDNA was identical to the protein. The amino acid sequence of the L. peruvianum fruit Inhibitor I exhibits 74% identity with the wound-inducible Inhibitor I from tomato leaves. Whereas all previously identified members of the Inhibitor I family have either Met, Leu, or Asp at the P1 site and can inhibit enzymes such as chymotrypsin, subtilisin, and elastase, the fruit Inhibitor I possesses Lys at the P1 position. Thus, this is the first member of the extensive Inhibitor I family from plants and animals that exhibits trypsin inhibitory specificity. The presence of this inhibitor in wild tomato fruit may reflect a functional role to protect the tissues against herbivory"
Keywords:		"Amino Acid Sequence Base Sequence DNA/*genetics/isolation & purification Genes, Plant Molecular Sequence Data Plant Development Plant Proteins/*genetics/isolation & purification Plants/*genetics *Protease Inhibitors Restriction Mapping Sequence Homology, ;"
Notes:		"MedlineWingate, V P Broadway, R M Ryan, C A eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 1989/10/25 J Biol Chem. 1989 Oct 25; 264(30):17734-8"