« Previous AbstractA deep learning approach for detecting liver cirrhosis from volatolomic analysis of exhaled breath    Next AbstractThe F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae »

Molecules

Title:		Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment
Author(s):		Wieczorek MN; Pieczywek PM; Cybulska J; Zdunek A; Jelen HH;
Address:		"Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland. Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin, Poland"
Journal Title:		Molecules
Year:		2022
Volume:		20220114
Issue:		2
Page Number:		-
DOI:		10.3390/molecules27020500
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) and sample pre-treatment methods, including the comminution technique or applied enzyme inhibition methods, could significantly influence the extracted volatile metabolome. Significant changes were observed in the volatile profile of broccoli florets frozen in liquid nitrogen at -20 degrees C. Those differences were mostly related to the concentration of nitriles and aldehydes. Confocal microscopy indicated some tissue deterioration in the case of slow freezing (-20 degrees C), whereas the structure of tissue, frozen in liquid nitrogen, was practically intact. Myrosinase activity assay proved that the enzyme remains active after freezing. No pH deviation was noted after sample storage - this parameter did not influence the activity of enzymes. Tissue fragmentation and enzyme-inhibition techniques applied prior to the extraction influenced both the qualitative and quantitative composition of the volatile metabolome of broccoli"
Keywords:		Brassica/growth & development/*metabolism Flowers/growth & development/*metabolism Food Handling/*methods Food Storage *Freezing Glycoside Hydrolases/*metabolism *Metabolome Plant Extracts/metabolism Plant Proteins/metabolism Volatile Organic Compounds/an;
Notes:		"MedlineWieczorek, Martyna N Pieczywek, Piotr Mariusz Cybulska, Justyna Zdunek, Artur Jelen, Henryk H eng 2015/18/M/NZ9/00372/National Science Center/ Switzerland 2022/01/22 Molecules. 2022 Jan 14; 27(2):500. doi: 10.3390/molecules27020500"