| Title: | Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p) |
| Author(s): | Wichmann H; Hengst L; Gallwitz D; |
| Address: | "Max Planck Institute for Biophysical Chemistry, Department of Molecular Genetics, Germany" |
| DOI: | 10.1016/s0092-8674(05)80062-5 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "From the budding yeast S. cerevisiae, we have cloned a gene, YPT7, that encodes a GTP-binding protein belonging to the Ypt family of ras-related proteins. The 208 amino acid protein shares identical effector domain and C-terminal sequences with the mammalian Rab7 protein. YPT7 gene disruption did not impair cellular growth at temperatures ranging from 17 degrees C to 37 degrees C. ypt7 null mutants are characterized by highly fragmented vacuoles and differential defects of vacuolar protein transport and maturation. The uptake of alpha factor pheromone by wild-type and Ypt7p-deficient cells was found to be indistinguishable, but in mutant cells lacking Ypt7p, degradation of the endocytosed pheromone was severely inhibited. Our findings suggest a role of Ypt7p in protein transport between endosome-like compartments" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Biological Transport DNA Mutational Analysis Endocytosis GTP-Binding Proteins/*genetics Genes, ras Molecular Sequence Data Proteins/metabolism Saccharomyces cerevisiae/*genetics *Saccharomyces cerevisiae Proteins;" |
| Notes: | "MedlineWichmann, H Hengst, L Gallwitz, D eng Research Support, Non-U.S. Gov't 1992/12/24 Cell. 1992 Dec 24; 71(7):1131-42. doi: 10.1016/s0092-8674(05)80062-5" |
