| Title: | Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p |
| Author(s): | Whiteway MS; Wu C; Leeuw T; Clark K; Fourest-Lieuvin A; Thomas DY; Leberer E; |
| Address: | "Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec" |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "The mating response pathway of the yeast Saccharomyces cerevisiae includes a heterotrimeric guanine nucleotide-binding protein (G protein) that activates a mitogen-activated protein MAP kinase cascade by an unknown mechanism. An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified. A haploid-specific interaction between the amino terminus of Ste5p and the G protein beta subunit Ste4p was also detected in a two-hybrid assay, and the product of a signaling-defective allele of STE4 was defective in this interaction. In cells with a constitutively activated pheromone response pathway, epitope-tagged Ste4p was coimmunoprecipitated with Ste5p. Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal" |
| Keywords: | "*Adaptor Proteins, Signal Transducing Base Sequence Calcium-Calmodulin-Dependent Protein Kinases/metabolism *Carrier Proteins Cell Division Fungal Proteins/genetics/*metabolism *GTP-Binding Protein beta Subunits *GTP-Binding Protein gamma Subunits GTP-Bin;" |
| Notes: | "MedlineWhiteway, M S Wu, C Leeuw, T Clark, K Fourest-Lieuvin, A Thomas, D Y Leberer, E eng Research Support, Non-U.S. Gov't 1995/09/15 Science. 1995 Sep 15; 269(5230):1572-5. doi: 10.1126/science.7667635" |
