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Proc Natl Acad Sci U S A

Title:		A cooperative model for receptor recognition and cell adhesion: evidence from the molecular packing in the 1.6-A crystal structure of the pheromone Er-1 from the ciliated protozoan Euplotes raikovi
Author(s):		Weiss MS; Anderson DH; Raffioni S; Bradshaw RA; Ortenzi C; Luporini P; Eisenberg D;
Address:		"Molecular Biology Institute, University of California, Los Angeles 90095-1570, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1995
Volume:		92
Issue:		22
Page Number:		10172 - 10176
DOI:		10.1073/pnas.92.22.10172
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"The crystal structure of the pheromone Er-1 from the unicellular eukaryotic organism Euplotes raikovi was determined at 1.6 A resolution and refined to a crystallographic R factor of 19.9%. In the tightly packed crystal, two extensive intermolecular helix-helix interactions arrange the Er-1 molecules into layers. Since the putative receptor of the pheromone is a membrane-bound protein, whose extracellular C-terminal domain is identical in amino acid sequence to the soluble pheromone, the interactions found in the crystal may mimic the pheromone-receptor interactions as they occur on a cell surface. Based on this, we propose a model for the interaction between soluble pheromone molecules and their receptors. In this model, strong pheromone-receptor binding emerges as a consequence of the cooperative utilization of several weak interactions. The model offers an explanation for the results of binding studies and may also explain the adhesion between cells that occurs during mating"
Keywords:		"Amino Acid Sequence Animals Cell Adhesion Crystallography, X-Ray Euplotes Macromolecular Substances Magnetic Resonance Spectroscopy Membrane Proteins/*chemistry/physiology Models, Molecular Pheromones/*chemistry *Protein Structure, Secondary Protozoan Pro;"
Notes:		"MedlineWeiss, M S Anderson, D H Raffioni, S Bradshaw, R A Ortenzi, C Luporini, P Eisenberg, D eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1995/10/24 Proc Natl Acad Sci U S A. 1995 Oct 24; 92(22):10172-6. doi: 10.1073/pnas.92.22.10172"