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Mol Cell Biol

Title:		Multimeric arrays of the yeast retrotransposon Ty
Author(s):		Weinstock KG; Mastrangelo MF; Burkett TJ; Garfinkel DJ; Strathern JN;
Address:		"Laboratory of Eukaryotic Gene Expression, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701"
Journal Title:		Mol Cell Biol
Year:		1990
Volume:		10
Issue:		6
Page Number:		2882 - 2892
DOI:		10.1128/mcb.10.6.2882-2892.1990
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"We have identified a novel integrated form of the yeast retrotransposon Ty consisting of multiple elements joined into large arrays. These arrays were first identified among Ty-induced alpha-pheromone-resistant mutants of MATa cells of Saccharomyces cerevisiae which contain Ty insertions at HML alpha that result in the expression of that normally silent cassette. These insertions are multimeric arrays of both the induced genetically marked Ty element and unmarked Ty elements. Structural analysis of the mutations indicated that the arrays include tandem direct repeats of Ty elements separated by only a single long terminal repeat. The Ty-HML junction fragments of one mutant were cloned and shown to contain a 5-base-pair duplication of the target sequence that is characteristic of a Ty transpositional insertion. In addition, the arrays include rearranged Ty elements that do not have normal long terminal repeat junctions. We have also identified multimeric Ty insertions at other chromosomal sites and as insertions that allow expression of a promoterless his3 gene on a plasmid. The results suggest that Ty transposition includes an intermediate that can undergo recombination to produce multimers"
Keywords:		"Bacteriophage lambda/genetics Base Sequence Blotting, Southern Centromere/ultrastructure Chromosomes, Fungal/ultrastructure Cloning, Molecular *DNA Transposable Elements DNA, Fungal/genetics Escherichia coli/genetics Gene Library *Genes, Fungal Molecular;"
Notes:		"MedlineWeinstock, K G Mastrangelo, M F Burkett, T J Garfinkel, D J Strathern, J N eng N01-CO-74101/CO/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1990/06/01 Mol Cell Biol. 1990 Jun; 10(6):2882-92. doi: 10.1128/mcb.10.6.2882-2892.1990"