| Title: | Establishment and application of a metabolomics workflow for identification and profiling of volatiles from leaves of Vitis vinifera by HS-SPME-GC-MS |
| Author(s): | Weingart G; Kluger B; Forneck A; Krska R; Schuhmacher R; |
| Address: | "Center for Analytical Chemistry, Department IFA-Tulln, University of Natural Resources and Life Sciences, Vienna, Konrad-Lorenz-Str. 20, 3430 Tulln, Austria" |
| ISSN/ISBN: | 1099-1565 (Electronic) 0958-0344 (Linking) |
| Abstract: | "INTRODUCTION: Volatile organic compounds (VOCs) occurring in leaves of plants carry information about the physiological state of the plant. Monitoring of VOCs assists in detecting plant stress before visible signs are present. OBJECTIVE: To establish and apply a simple workflow for the automated extraction, measurement and annotation/identification of Vitis vinifera cv. Pinot Noir leaf metabolites. METHODOLOGY: Leaf samples were harvested, cooled with liquid nitrogen and homogenised under cooled conditions. VOCs were extracted and enriched by solid phase microextraction (SPME) and analysed by GC-MS. Samples were measured on two columns with different polarity of stationary phases. Mass spectral deconvolution and identification was done by AMDIS software. Strict identification criteria were applied: match factor >/= 90; relative retention index deviation |
| Keywords: | Gas Chromatography-Mass Spectrometry Metabolome Metabolomics/*methods Plant Leaves/*chemistry Principal Component Analysis Reproducibility of Results *Software Solid Phase Microextraction/*methods Vitis/*chemistry Volatile Organic Compounds/chemistry/*iso; |
| Notes: | "MedlineWeingart, Georg Kluger, Bernhard Forneck, Astrid Krska, Rudolf Schuhmacher, Rainer eng Research Support, Non-U.S. Gov't England 2011/10/20 Phytochem Anal. 2012 Jul-Aug; 23(4):345-58. doi: 10.1002/pca.1364. Epub 2011 Oct 18" |
