Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous Abstract"Odorant and pheromone binding by aphrodisin, a hamster aphrodisiac protein"    Next AbstractIsotopic double-labeling of two honeybee odorant-binding proteins secreted by the methylotrophic yeast Pichia pastoris »

J Pept Res


Title:"Disulfide pairing and secondary structure of ASP1, an olfactory-binding protein from honeybee (Apis mellifera L)"
Author(s):Briand L; Nespoulous C; Huet JC; Pernollet JC;
Address:"Biochimie et Structure des Proteines, INRA, Jouy-en-Josas, France"
Journal Title:J Pept Res
Year:2001
Volume:58
Issue:6
Page Number:540 - 545
DOI: 10.1034/j.1399-3011.2001.00949.x
ISSN/ISBN:1397-002X (Print) 1397-002X (Linking)
Abstract:"In insects, the transport of airborne, hydrophobic odorants and pheromones through the sensillum lymph is accomplished by olfactory-binding proteins (CBPs). We report the structural characterization of a honeybee OBP called ASP1 found in workers and drones, previously observed to bind queen pheromone components. A novel method based on ion-spray mass spectrometry analysis of cyanylation-induced cleavage products of partially reduced protein with Tris(2-carboxyethyl)phosphine was needed to determine the recombinant ASP1 disulfide bond pairing. It was observed to be Cys(I)-Cys(III), Cys(II)-Cys(V), Cys(IV)-Cys(VI), similar to those already described for other OBPs from honeybee and Bombyx mori suggesting that this pattern occurs commonly throughout the diverse family of insect OBPs. Circular dichroism revealed that ASP1 is an all-alpha protein in accordance with NMR preliminary data, but unlike lipocalin-like vertebrate OBPs"
Keywords:"Animals Bees Carrier Proteins/*chemistry Circular Dichroism Disulfides/*chemistry Insect Proteins/*chemistry Protein Structure, Secondary;"
Notes:"MedlineBriand, L Nespoulous, C Huet, J C Pernollet, J C eng Research Support, Non-U.S. Gov't Denmark 2002/05/15 J Pept Res. 2001 Dec; 58(6):540-5. doi: 10.1034/j.1399-3011.2001.00949.x"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 24-11-2024