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Environ Entomol

Title:		Enhanced beta-ionone emission in Arabidopsis over-expressing AtCCD1 reduces feeding damage in vivo by the crucifer flea beetle
Author(s):		Wei S; Hannoufa A; Soroka J; Xu N; Li X; Zebarjadi A; Gruber M;
Address:		"College of Tea & Food Sciences, Anhui Agricultural University, Hefei, 230036, China"
Journal Title:		Environ Entomol
Year:		2011
Volume:		40
Issue:		6
Page Number:		1622 - 1630
DOI:		10.1603/EN11088
ISSN/ISBN:		1938-2936 (Electronic) 0046-225X (Linking)
Abstract:		"Plant carotenoid derived beta-ionone has been shown to have diverse biological effects on some insect herbivores and herbivore parasitoids. In this study, Arabidopsis transgenic plants over-expressing a carotenoid cleavage dioxygenase1 gene (AtCCD1) were generated to test whether beta-ionone emissions could be enhanced and used to control feeding by the crucifer flea beetle (Phyllotreta cruciferae Goeze). The transgenic plants exhibited a morphological phenotype indistinguishable from the wild type (WT) control over their complete life cycle. Gas chromatography and mass spectrometry analyses of headspace volatiles collected from 6-wk-old intact flowering plants revealed substantially enhanced beta-ionone emission from transgenic plants compared with WT, but no beta-ionone enhancement occurred at a young vegetative stage (4-wk-old seedlings). Bioassays in an enclosed environment showed that AtCCD1 over-expression resulted in asymptotically equal to50% less leaf area damage by flea beetles on transgenic plants compared with WT plants. The mean number of damaged transgenic leaves per plant also was significantly lower in transgenic plants (P<0.05). Our results indicate that AtCCD1 over-expression and induced beta-ionone emission might find application in the control of pests for Brassica crops grown in greenhouse operations. Potentially, beta-ionone also could be used on crops grown in open-air ecosystems if this allomone is released in sufficient quantities to discourage herbivore foragers"
Keywords:		"Animals Arabidopsis/genetics/*metabolism Arabidopsis Proteins/genetics/metabolism Chromatography, Gas Coleoptera/*drug effects/physiology Dioxygenases/genetics/metabolism Herbivory Mass Spectrometry Norisoprenoids/metabolism Plants, Genetically Modified/g;"
Notes:		"MedlineWei, Shu Hannoufa, Abdelali Soroka, Julie Xu, Ning Li, Xiang Zebarjadi, Alireza Gruber, Margaret eng England 2012/01/06 Environ Entomol. 2011 Dec; 40(6):1622-30. doi: 10.1603/EN11088"