| Title: | Effects of a Volatile Organic Compound Filter on Breath Profiles Measured by Secondary Electrospray High-Resolution Mass Spectrometry |
| Author(s): | Weber R; Kaeslin J; Moeller S; Perkins N; Micic S; Moeller A; |
| Address: | "Department of Respiratory Medicine and Childhood Research Center, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032 Zurich, Switzerland. Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Vladimir-Prelog Weg 1-5/10, 8093 Zurich, Switzerland. Division of Clinical Chemistry and Biochemistry, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032 Zurich, Switzerland. Faculty of Medicine, University of Zurich, Raemistrasse 71, 8006 Zurich, Switzerland" |
| DOI: | 10.3390/molecules28010045 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Environmental volatile organic compounds (VOCs) from the ambient air potentially influence on-line breath analysis measurements by secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS). The aim of this study was to investigate how inhaling through a VOC filter affects the detected breath profiles and whether it is feasible to integrate such filters into routine measurements. A total of 24 adult participants performed paired breath analysis measurements with and without the use of an activated carbon filter for inspiration. Concordance correlation coefficients (CCCs) and the Bland-Altman analysis were used to assess the agreement between the two methods. Additionally, the effect on a selection of known metabolites and contaminants was analyzed. Out of all the detected features, 78.3% showed at least a moderate agreement before and after filter usage (CCC > 0.9). The decrease in agreement of the remaining m/z features was mostly associated with reduced signal intensities after filter usage. Although a moderate-to-substantial concordance was found for almost 80% of the m/z features, the filter still had an effect by decreasing signal intensities, not only for contaminants, but also for some of the studied metabolites. Operationally, the use of the filter complicated and slowed down the conductance of measurements, limiting its applicability in clinical studies" |
| Keywords: | "Adult Humans *Volatile Organic Compounds/analysis Spectrometry, Mass, Electrospray Ionization/methods Breath Tests/methods Air/analysis Charcoal Sesi-hrms ambient air contaminants filter metabolites volatile organic compounds;" |
| Notes: | "MedlineWeber, Ronja Kaeslin, Jerome Moeller, Sophia Perkins, Nathan Micic, Srdjan Moeller, Alexander eng 326030_177101/1/SNSF_/Swiss National Science Foundation/Switzerland Evi Diethelm-Winteler Foundation/ Childhood Research Center of the University Children's Hospital Zurich/ Switzerland 2023/01/09 Molecules. 2022 Dec 21; 28(1):45. doi: 10.3390/molecules28010045" |
