« Previous AbstractConstruction of Enterococcus faecalis pAD1 miniplasmids: identification of a minimal pheromone response regulatory region and evaluation of a novel pheromone-dependent growth inhibition    Next Abstract"Identification, characterization, and nucleotide sequence of a region of Enterococcus faecalis pheromone-responsive plasmid pAD1 capable of autonomous replication" »

Plasmid

Title:		Control of Enterococcus faecalis sex pheromone cAD1 elaboration: effects of culture aeration and pAD1 plasmid-encoded determinants
Author(s):		Weaver KE; Clewell DB;
Address:		"Department of Microbiology, School of Medicine, University of South Dakota, Vermillion 57069"
Journal Title:		Plasmid
Year:		1991
Volume:		25
Issue:		3
Page Number:		177 - 189
DOI:		10.1016/0147-619x(91)90011-k
ISSN/ISBN:		0147-619X (Print) 0147-619X (Linking)
Abstract:		"Aeration of plasmid-free Enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cAD1 activity in culture filtrates. Levels of two unrelated pheromones, cPD1 and cAM373, were unaffected by culture aeration. Aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pAD1 traB mutations, verifying a link between traB function and pheromone 'shutdown.' Tests with a series of pAD1 mini-plasmids indicated that the product of the traB gene was involved in, but not sufficient for, pheromone shutdown; the cooperation of one or more other gene products encoded within the pheromone response control region was required"
Keywords:		"Aerobiosis Conjugation, Genetic Enterococcus faecalis/cytology/*genetics/physiology Genes, Bacterial Genotype Oligopeptides/*genetics Phenotype *Plasmids Restriction Mapping beta-Galactosidase/genetics/metabolism;"
Notes:		"MedlineWeaver, K E Clewell, D B eng AI10318/AI/NIAID NIH HHS/ GM33956/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1991/05/01 Plasmid. 1991 May; 25(3):177-89. doi: 10.1016/0147-619x(91)90011-k"