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Anal Chem

Title:		GC/MS with post-column switching for large volume injection of headspace samples: sensitive determination of volatile organic compounds in human whole blood and urine
Author(s):		Watanabe K; Fujita H; Hasegawa K; Gonmori K; Suzuki O;
Address:		"Department of Legal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan. Kanako@hama-med.ac.jp"
Journal Title:		Anal Chem
Year:		2011
Volume:		20110126
Issue:		4
Page Number:		1475 - 1479
DOI:		10.1021/ac1026258
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"When volatile or semivolatile compounds are measured by headspace (HS) gas chromatography (GC)/mass spectrometry (MS), the maximum gas volume to be injected is usually 0.5-1.0 mL; over the volume, the MS detector automatically shuts down due to impairment of the vacuum rate of the MS ionization chamber. To overcome the problem, we modified the gas flow routes of a new type of GC/MS instrument to create a postcolumn switching system, which can eliminate the large volume of gas before introduction of target compounds into the MS ionization chamber. Our HS-GC/MS system enabled injection of as large as 5 mL of HS gas without any disturbance. As the first example analysis, we tried to establish the analysis of naphthalene and p-dichlorobenzene in human whole blood and urine by this method with large volume injection. The limits of detection for both compounds in whole blood and urine were as low as about 10 and 5 pg/mL, respectively. The validation data and actual measurements were also demonstrated. The new GC/MS system has great potential to analyze any type of volatile or semivolatile organic compounds in biological matrixes with very high sensitivity and full automation"
Keywords:		Blood Chemical Analysis/*methods Gas Chromatography-Mass Spectrometry/*methods Gases/chemistry Humans Injections Reproducibility of Results Urinalysis/*methods Volatile Organic Compounds/*blood/*urine;
Notes:		"MedlineWatanabe, Kanako Fujita, Hiroki Hasegawa, Koutaro Gonmori, Kunio Suzuki, Osamu eng 2011/01/28 Anal Chem. 2011 Feb 15; 83(4):1475-9. doi: 10.1021/ac1026258. Epub 2011 Jan 26"