| Title: | Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress |
| Author(s): | Wang XZ; Liu SS; Sun Y; Wu JY; Zhou YL; Zhang JH; |
| Address: | "Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing 400716, China" |
| DOI: | 10.1016/j.theriogenology.2009.04.016 |
| ISSN/ISBN: | 1879-3231 (Electronic) 0093-691X (Linking) |
| Abstract: | "Cypermethrin (CYP), an insecticide, has deleterious effects on male reproductive function. The objective was to identify whether the effects of beta-CYP on male reproductive organs were associated with oxidative stress. Three doses of beta-CYP (1, 10, and 20mg/kg) were administered to male mice for 35 d, with or without vitamin E (20mg/kg). The moderate (10mg/kg) and high (20mg/kg) doses of beta-CYP not only decreased body weight and the weight of the testes, epididymides, seminal vesicles, and prostate (P<0.05) but also reduced serum testosterone concentration and the expression of steroidogenic acute regulatory protein (P<0.05), in addition to damaging the seminiferous tubules and sperm development. Furthermore, moderate and high doses of beta-CYP administration decreased sperm number, sperm motility, and intact acrosome rate (P<0.05). Based on ultrastructural analyses, high doses of beta-CYP produced swelling and degeneration of mitochondria and the smooth endoplasmic reticulum of Leydig cells and caused the formation of concentric circles. These toxic effects of beta-CYP may be mediated by increasing oxidative stress, as the moderate and high doses of this compound increased malondialdehyde and nitric oxide in testes (P<0.05); reduced the activity of catalase, glutathione peroxidase (GSH-Px), and superoxide dismutase (P<0.05); and activated ERK1/2 (P<0.05). Vitamin E reversed the effects of beta-CYP on testosterone production and testis damage (P<0.05 vs. the high-dose group). Therefore, we inferred that beta-CYP damaged the structure of testes and decreased sperm output by inducing oxidative stress" |
| Keywords: | "Animals Animals, Outbred Strains Epididymis/anatomy & histology/drug effects/metabolism Insecticides/toxicity Leydig Cells/cytology/drug effects/ultrastructure Lipid Peroxidation/drug effects Male Mice Organ Size/drug effects Oxidative Stress/*drug effect;" |
| Notes: | "MedlineWang, X-Z Liu, S-S Sun, Y Wu, J-Y Zhou, Y-L Zhang, J-H eng Evaluation Study Research Support, Non-U.S. Gov't 2009/06/09 Theriogenology. 2009 Sep 15; 72(5):599-611. doi: 10.1016/j.theriogenology.2009.04.016. Epub 2009 Jun 4" |
