| Title: | Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery |
| Author(s): | Wang X; Pai CY; Stone DE; |
| Address: | "Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL. Howard Hughes Medical Institute, Department of Developmental Biology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA" |
| ISSN/ISBN: | 1540-8140 (Electronic) 0021-9525 (Print) 0021-9525 (Linking) |
| Abstract: | "The mating of budding yeast depends on chemotropism, a fundamental cellular process. Haploid yeast cells of opposite mating type signal their positions to one another through mating pheromones. We have proposed a deterministic gradient sensing model that explains how these cells orient toward their mating partners. Using the cell-cycle determined default polarity site (DS), cells assemble a gradient tracking machine (GTM) composed of signaling, polarity, and trafficking proteins. After assembly, the GTM redistributes up the gradient, aligns with the pheromone source, and triggers polarized growth toward the partner. Since positive feedback mechanisms drive polarized growth at the DS, it is unclear how the GTM is released for tracking. What prevents the GTM from triggering polarized growth at the DS? Here, we describe two mechanisms that are essential for tracking: inactivation of the Ras GTPase Bud1 and positioning of actin-independent vesicle delivery upgradient" |
| Keywords: | Actins/metabolism Cell Polarity/physiology Pheromones/metabolism *Saccharomyces cerevisiae/metabolism *Saccharomyces cerevisiae Proteins/genetics/metabolism *Transport Vesicles *rab GTP-Binding Proteins/genetics/metabolism ras Proteins/metabolism; |
| Notes: | "MedlineWang, Xin Pai, Chih-Yu Stone, David E eng Research Support, U.S. Gov't, Non-P.H.S. 2022/09/27 J Cell Biol. 2022 Dec 5; 221(12):e202203004. doi: 10.1083/jcb.202203004. Epub 2022 Sep 26" |
