| Title: | Dietary fermented soybean meal replacement alleviates diarrhea in weaned piglets challenged with enterotoxigenic Escherichia coli K88 by modulating inflammatory cytokine levels and cecal microbiota composition |
| Author(s): | Wang W; Wang Y; Hao X; Duan Y; Meng Z; An X; Qi J; |
| Address: | "College of Animal Science, Inner Mongolia Agricultural University, 010018, Hohhot, China. Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, 010018, Hohhot, China. College of Animal Science, Inner Mongolia Agricultural University, 010018, Hohhot, China. wangyuan@imau.edu.cn. Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, 010018, Hohhot, China. wangyuan@imau.edu.cn. College of Animal Science, Inner Mongolia Agricultural University, 010018, Hohhot, China. qijignwei_66@126.com. Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, 010018, Hohhot, China. qijignwei_66@126.com" |
| DOI: | 10.1186/s12917-020-02466-5 |
| ISSN/ISBN: | 1746-6148 (Electronic) 1746-6148 (Linking) |
| Abstract: | "BACKGROUND: Impaired gut microbiota leads to pathogenic bacteria infection, pro-inflammatory response and post-weaning diarrhea. Enterotoxigenic Escherichia coli (ETEC) K88 is a major cause of post-weaning diarrhea in weaned piglets. Fermented soybean meal (FSBM) could relieve diarrhea, alleviate inflammatory response, and modulate gut microbiota of weaned piglets. We used ETEC K88-challenged weaned piglet model to investigate the effects of FSBM on the growth performance, inflammatory response and cecal microbiota. Twenty-four crossbred piglets (6.8 +/- 0.5 kg; 21 +/- 2 days of age) were allotted into 2 treatment fed the diets with or without FSBM (6% at the expense of soybean meal). Six weaned piglets in each diet treatment were challenged by ETEC K88 (1 x 10(9) CFU/piglets) on day 15. The experimental period lasted for 20 days. RESULTS: The ETEC K88 challenge decreased (p < 0.05) fecal consistency and plasma interleukin-10 (IL-10) concentration, while increased (p < 0.05) average daily feed intake (ADFI) and plasma tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin 6 (IL-6) concentrations. After ETEC K88 challenge, dietary FSBM replacement increased (p < 0.05) final body weight (BW), average daily gain (ADG), ADFI, and fecal consistency, but decreased feed conversion ratio (FCR). The plasma IL-10 concentration of weaned piglets fed FSBM was higher (p < 0.05), while IL-1beta, IL-6 and TNF-alpha concentrations were lower (p < 0.05). Dietary FSBM replacement attenuated the increase of plasma TNF-alpha concentration and the decrease of ADG induced by ETEC K88 challenge (p < 0.05). High-throughput sequencing of 16S rRNA gene V4 region of cecal microbiota revealed that ETEC K88 challenge increased (p < 0.05) Campylobacter relative abundance on genus level. Dietary FSBM replacement resulted in higher (p < 0.05) relative abundances of Bacteroidetes and Prevotellaceae_NK3B31_group, and lower (p < 0.05) relative abundances of Proteobacteria and Actinobacillus. Furthermore, dietary FSBM replacement relieved the increase of Escherichia-Shigella relative abundance in weaned piglets challenged by ETEC K88 (p < 0.05). CONCLUSIONS: Dietary FSBM replacement improved growth performance and alleviated the diarrhea of weaned piglets challenged with ETEC K88, which could be due to modulation of cecal microbiota composition and down-regulation of inflammatory cytokines production" |
| Keywords: | Animal Feed/*analysis Animals Bacteria/classification Cecum/microbiology Cytokines/blood Diarrhea/diet therapy/microbiology/veterinary Diet/veterinary Enterotoxigenic Escherichia coli Escherichia coli Infections/diet therapy/microbiology/*veterinary Femal; |
| Notes: | "MedlineWang, Wenwen Wang, Yuan Hao, Xiran Duan, Yuanxiao Meng, Ziqi An, Xiaoping Qi, Jingwei eng 31601969/National Natural Science Foundation of China/ NDSC2018-04/IMAU 'Double-First Class' Innovation Group Personnel Training Project/ 2018/Science and Technology Planning Project of Inner Mongolia/ C2016036/project for Mr. Yang Sheng protege community/ England 2020/07/16 BMC Vet Res. 2020 Jul 14; 16(1):245. doi: 10.1186/s12917-020-02466-5" |
