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J Insect Physiol

Title:		A novel cytochrome P450 CYP6AB14 gene in Spodoptera litura (Lepidoptera: Noctuidae) and its potential role in plant allelochemical detoxification
Author(s):		Wang RL; Xia QQ; Baerson SR; Ren Y; Wang J; Su YJ; Zheng SC; Zeng RS;
Address:		"Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China. United States Department of Agriculture-Agricultural Research Service, Natural Products Utilization Research Unit, University, MS 38677, USA. School of Life Sciences, South China Normal University, Guangzhou 510631, China. Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: rszeng@scau.edu.cn"
Journal Title:		J Insect Physiol
Year:		2015
Volume:		20150314
Issue:
Page Number:		54 - 62
DOI:		10.1016/j.jinsphys.2015.02.013
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"Cytochrome P450 monooxygenases (P450s) play a prominent role in the adaptation of insects to host plant chemical defenses. To investigate the potential role of P450s in adaptation of the lepidopteran pest Spodoptera litura to host plant allelochemicals, an expressed sequence data set derived from 6th instar midgut tissues was first mined. One sequence identified from the S. litura 6th instar midgut EST database was determined by phylogenetic analysis to belong to the CYP6AB P450 subfamily, and named CYP6AB14. Dietary supplementation of S. litura larvae with either xanthotoxin (XAN), coumarin (COU) and flavone (FLA) led to elevated CYP6AB14 transcript levels in both midgut and fat body tissues. Injection of CYP6AB14-derived double-stranded RNA (dsRNA) into S. litura individuals significantly reduced CYP6AB14 transcript levels, and resulted in increased developmental abnormalities and higher mortality rates among XAN, COU and FLA-fed larvae. Our results strongly suggest a key role for CYP6AB14 in plant allelochemical detoxification in S. litura"
Keywords:		Amino Acid Sequence Animals Coumarins/toxicity Cytochrome P-450 Enzyme System/*genetics/metabolism Digestive System/enzymology Enzyme Induction Flavones/toxicity Insect Proteins/*genetics/metabolism Larva/drug effects/metabolism Methoxsalen/toxicity Molec;
Notes:		"MedlineWang, Rui-Long Xia, Qing-Qing Baerson, Scott R Ren, Yong Wang, Jie Su, Yi-Juan Zheng, Si-Chun Zeng, Ren-Sen eng Research Support, Non-U.S. Gov't England 2015/03/19 J Insect Physiol. 2015 Apr; 75:54-62. doi: 10.1016/j.jinsphys.2015.02.013. Epub 2015 Mar 14"