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Insect Biochem Mol Biol

Title:		Coordinative mediation of the response to alarm pheromones by three odorant binding proteins in the green peach aphid Myzus persicae
Author(s):		Wang Q; Liu JT; Zhang YJ; Chen JL; Li XC; Liang P; Gao XW; Zhou JJ; Gu SH;
Address:		"Department of Entomology, China Agricultural University, Beijing, 100193, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; College of Plant Protection, Shenyang Agricultural University, Shenyang, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Department of Entomology and BIO5 Institute, University of Arizona, Tucson, USA. Department of Entomology, China Agricultural University, Beijing, 100193, China. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, China; State Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang, China. Department of Entomology, China Agricultural University, Beijing, 100193, China. Electronic address: gushaohua@cau.edu.cn"
Journal Title:		Insect Biochem Mol Biol
Year:		2021
Volume:		20210119
Issue:
Page Number:		103528 -
DOI:		10.1016/j.ibmb.2021.103528
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Odorant binding proteins (OBPs) play an essential role for insect chemosensation in insect peripheral nervous systems of antennae. Each antennal sensilla contains more than one OBP at high concentrations but the interactions and cooperation between co-localized OBPs are rarely reported. In present study, we cloned, expressed and purified eight OBPs of the green peach aphid Myzus persicae. The effects of knocking down the expression of these OBP genes by RNAi on the electrophysiological and behavioural responses of M. persicae to the aphid alarm pheromone, (E)-beta-farnesene (EbetaF) were investigated. The results showed that the aphids could still be repelled by EbetaF when the expression of each of three OBP genes was individually knocked down. However, the simultaneous knockdown of MperOBP3/7/9 expression significantly reduced the electrophysiological response and the repellent behaviours of M. persicae to EbetaF than the single OBP gene knockdown (P < 0.05). Rather than a normal saturation binding curve of individual OBP, the binding curve of MperOBP3/7/9 is bell-shaped with a higher affinity for the fluorescent probe N-phenyl-1-naphthylamine (1-NPN). The competitive binding assays confirmed that MperOBP3, MperOBP7, MperOBP9 and MperOBP3/7/9 mixture exhibited a stronger binding affinity for EbetaF, than for sex pheromones and plant volatiles with a dissociation constant of 2.5 muM, 1.1 muM, 3.9 muM and 1.0 muM, respectively. The competitive binding curve of MperOBP3/7/9 mixture to EbetaF is shallow without bottom plateau, suggesting a conformational change and a rapid dissociation after the displacement of all 1-NPN (in vivo after the saturation binding of all OBPs by EbetaF). The interaction between OBPs and formation of a heterogeneous unit may facilitate the delivery EbetaF to the OR at electrophysiological and behavioural levels during insect odorant signal transduction thus mediate M. persicae response to the alarm pheromone EbetaF"
Keywords:		"Animals *Aphids/genetics/metabolism/physiology Behavior, Animal Electrophysiology/methods Gene Silencing Genes, Insect Insect Proteins/biosynthesis/drug effects/genetics/metabolism Odorants Pheromones/pharmacology Phylogeny RNA Interference *Receptors, Od;"
Notes:		"MedlineWang, Qian Liu, Jing-Tao Zhang, Yong-Jun Chen, Ju-Lian Li, Xian-Chun Liang, Pei Gao, Xi-Wu Zhou, Jing-Jiang Gu, Shao-Hua eng Research Support, Non-U.S. Gov't England 2021/01/23 Insect Biochem Mol Biol. 2021 Mar; 130:103528. doi: 10.1016/j.ibmb.2021.103528. Epub 2021 Jan 19"